Wang Yu, Lv Yang, Gao Shujun, Zhang Yuanyuan, Sun Jiajia, Gong Chunling, Chen Xiujuan, Li Guangpeng
The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China.
Department of Gynecology and Obstetrics, Inner Mongolia Medical University affiliated hospital, Hohhot, China.
PLoS One. 2016 Jan 6;11(1):e0143116. doi: 10.1371/journal.pone.0143116. eCollection 2016.
To comparatively analyze the human microRNA (miRNA) profiles between spontaneous decidualized menstrual endometrium and early pregnancy decidua by an in-depth sequencing of miRNAs. The specific miRNAs expressed at conception might be involved in pregnancy establishment and expression of let-7f-5p and let-7g-5p was experimentally up-regulated or inhibited to assess the effect on the expression of IGF2BP-1 and IGF2R in vitro, respectively. Samples of endometria and deciduas were obtained from 25 women who suffered from tubal or male factor subfertility and from 35 early pregnant women who underwent pregnancy termination at 6-8 weeks gestation were irrespectively collected and comparatively analyzed by miRNA sequencing and differential expression of known and novel miRNAs was analyzed using bioinformatics. The 2042 miRNA expression was analyzed in the study and the differential expression of six miRNAs was validated by qRT-PCR. The expression of four miRNAs in decidua samples was down-regulated (miR-34c, miR-92a, miR-181a-5p, and miR-191), whereas the expression of miR-10a-5p and let-7f-5p was significantly up-regulated. The expression of IGF2BP-1 and IGF2R declined and increased with overexpression and inhibition of let-7f-5p and let-7g-5p, respectively. Changes in the expression of particular miRNAs might play a role in the physiology of decidualization following successful embryo implantation, ultimately resulting in continuous decidualization.
通过对微小RNA（miRNA）进行深度测序，比较分析自然蜕膜化的月经子宫内膜与早孕蜕膜之间的人类miRNA谱。受孕时表达的特定miRNA可能参与妊娠建立，分别对let-7f-5p和let-7g-5p的表达进行实验性上调或抑制，以评估其对体外IGF2BP-1和IGF2R表达的影响。分别收集了25例因输卵管或男性因素导致的亚生育妇女的子宫内膜和蜕膜样本，以及35例在妊娠6-8周时接受终止妊娠的早孕妇女的样本，通过miRNA测序进行比较分析，并使用生物信息学分析已知和新型miRNA的差异表达。本研究分析了2042个miRNA的表达，并通过qRT-PCR验证了6个miRNA的差异表达。蜕膜样本中4个miRNA的表达下调（miR-34c、miR-92a、miR-181a-5p和miR-191），而miR-10a-5p和let-7f-5p的表达显著上调。IGF2BP-1和IGF2R的表达分别随着let-7f-5p和let-7g-5p的过表达和抑制而下降和增加。特定miRNA表达的变化可能在胚胎成功着床后蜕膜化的生理过程中起作用，最终导致持续蜕膜化。
