参与甲型禽流感（H10N7）病毒在小鼠体内适应性的多个氨基酸替换。
Multiple amino acid substitutions involved in the adaptation of avian-origin influenza A (H10N7) virus in mice.
作者信息
Wu Haibo, Peng Xiuming, Peng Xiaorong, Cheng Linfang, Jin Changzhong, Lu Xiangyun, Xie Tiansheng, Yao Hangping, Wu Nanping
机构信息
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
出版信息
Arch Virol. 2016 Apr;161(4):977-80. doi: 10.1007/s00705-015-2722-6. Epub 2015 Dec 23.
To identify substitutions that are possibly associated with the adaptation of avian-origin H10N7 virus to mammals, adaptation of the H10N7 virus in mouse lung was carried out by serial lung-to-lung passage. Genomic analysis of the mouse-adapted virus revealed amino acid changes in the PB2 (E627K), PA (T97I), and HA (G409E) proteins, and this virus was more virulent in mice than the wild-type virus. Our results suggest that these substitutions are involved in the enhancement of the replication efficiency of avian-origin H10N7 virus, resulting in severe disease in mice. Continued poultry surveillance of these substitutions in H10N7 viruses is required.
为了鉴定可能与禽源H10N7病毒适应哺乳动物相关的替换，通过连续的肺对肺传代在小鼠肺中进行H10N7病毒的适应。对适应小鼠的病毒进行基因组分析，发现PB2（E627K）、PA（T97I）和HA（G409E）蛋白中的氨基酸发生了变化，并且该病毒在小鼠中比野生型病毒更具致病性。我们的结果表明，这些替换参与了禽源H10N7病毒复制效率的提高，导致小鼠出现严重疾病。需要持续对H10N7病毒中的这些替换进行家禽监测。
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