Efimtsev E I, Shtannikova E V
Nauchnye Doki Vyss Shkoly Biol Nauki. 1989(5):85-9.
The fluorescence spectra of Bacillus thuringiensis native cells at 340 nm induction have been investigated. The spectral curve has 440-445 and 510-515 nm maximums of fluorescence of pyridine nucleotides (PN) and flavoproteins (FP). The induction of PN and FP fluorescence under anaerobiosis resulted from bacterium breath has been found. The fluorescence induction is connected with the viability of cells. The amplitude of induced fluorescence is in a linear dependence on the number of vegetative cells in medium. The given experimental approaches may be used in a course of development of methods of continuous control over the physiological state change of microorganisms.
研究了苏云金芽孢杆菌天然细胞在340nm诱导下的荧光光谱。光谱曲线在440 - 445nm和510 - 515nm处分别有吡啶核苷酸（PN）和黄素蛋白（FP）的最大荧光值。已发现厌氧菌呼吸导致的PN和FP荧光诱导现象。荧光诱导与细胞活力相关。诱导荧光的幅度与培养基中营养细胞的数量呈线性关系。所给出的实验方法可用于微生物生理状态变化连续控制方法的开发过程。
