van Zelst Mariëlle, Hesta Myriam, Gray Kerry, Janssens Geert P J
Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke, Belgium.
WALTHAM Centre for Pet Nutrition, Freeby Lane, Waltham-on-the-Wolds, Leicestershire, UK.
Biol Trace Elem Res. 2016 Aug;172(2):361-363. doi: 10.1007/s12011-015-0603-1. Epub 2015 Dec 23.
Glutathione peroxidase activity is used as a biomarker of selenium status in dogs. Freshly collected blood samples are usually measured, due to the lack of knowledge on the effect of storing the samples. This study investigated if the analysis of glutathione peroxidase activity in whole blood collected from dogs was affected by storage of between 5 and 164 days. Results indicated that glutathione peroxidase activity was more variable in the freshly analysed samples compared to the stored samples. Although the mean differences between fresh and stored samples were not always equal to zero, this is thought to be caused by the variability of reagent preparation rather than by storage, as no consistent increase or decrease in glutathione peroxidase activity was found. Therefore, it can be concluded that heparinised dog blood samples can be successfully stored up to 164 days before analysis of glutathione peroxidase activity.
谷胱甘肽过氧化物酶活性被用作犬类硒状态的生物标志物。由于对样本储存影响的了解不足，通常对新鲜采集的血液样本进行检测。本研究调查了犬全血中谷胱甘肽过氧化物酶活性分析是否会受到5至164天储存时间的影响。结果表明，与储存样本相比，新鲜分析的样本中谷胱甘肽过氧化物酶活性的变异性更大。虽然新鲜样本和储存样本之间的平均差异并不总是等于零，但这被认为是由试剂制备的变异性而非储存导致的，因为未发现谷胱甘肽过氧化物酶活性有持续增加或降低的情况。因此，可以得出结论，在分析谷胱甘肽过氧化物酶活性之前，肝素化犬血样本可成功储存长达164天。
