Kayaaltı Zeliha, Sert Selda, Kaya-Akyüzlü Dilek, Söylemez Esma, Söylemezoğlu Tülin
Institute of Forensic Sciences, Ankara University, Dikimevi, 06590 Ankara, Turkey.
Institute of Forensic Sciences, Ankara University, Dikimevi, 06590 Ankara, Turkey.
Environ Toxicol Pharmacol. 2016 Jan;41:147-51. doi: 10.1016/j.etap.2015.11.017. Epub 2015 Dec 3.
Lead inhibits the delta-aminolevulinic acid dehydratase (ALAD) activity and results in neurotoxic aminolevulinic acid accumulation in the blood. During pregnancy, lead in the maternal blood can easily cross the placenta. The aim of this study was to determine whether the maternal ALAD G177C polymorphism (rs1800435) was related to the placental lead levels. The study population comprised 97 blood samples taken from mothers to investigate ALAD G177C polymorphism and their placentas to measure lead levels. ALAD G177C polymorphism was detected by standard polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) technique and atomic absorption spectrometry (AAS) equipped with a graphite furnace and Zeeman background correction system was used for lead determination. The median placental lead levels for ALAD1-1, ALAD1-2 and ALAD2-2 genotypes were 7.54 μg/kg, 11.78 μg/kg and 18.53 μg/kg, respectively. Statistically significant association was found between the maternal ALAD G177C polymorphism and placental lead levels (p<0.05). This study suggested that maternal ALAD G177C polymorphism was associated with placental lead levels.
铅会抑制δ-氨基乙酰丙酸脱水酶（ALAD）的活性，并导致具有神经毒性的氨基乙酰丙酸在血液中蓄积。孕期，母体血液中的铅很容易穿过胎盘。本研究的目的是确定母体ALAD基因G177C多态性（rs1800435）是否与胎盘铅水平相关。研究对象包括97份取自母亲的血样，用于调查ALAD基因G177C多态性，以及她们的胎盘，用于测量铅水平。采用标准聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）技术检测ALAD基因G177C多态性，使用配备石墨炉和塞曼背景校正系统的原子吸收光谱法（AAS）测定铅含量。ALAD1-1、ALAD1-2和ALAD2-2基因型的胎盘铅水平中位数分别为7.54μg/kg、11.78μg/kg和18.53μg/kg。母体ALAD基因G177C多态性与胎盘铅水平之间存在统计学显著关联（p<0.05）。本研究表明，母体ALAD基因G177C多态性与胎盘铅水平有关。
