Azuma M, Kawamata H, Kasai Y, Yanagawa T, Yoshida H, Sato M
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
Cancer Res. 1989 Oct 1;49(19):5435-42.
A clonal cell with an acinar cell phenotype, which was induced by 5-azacytidine treatment of a neoplastic human salivary intercalated duct cell line, was cultivated in the presence of 1 alpha,25-dihydroxyvitamin D3. Morphological changes occurred; large cells that were polygonal or round in shape and had numerous vacuoles in their cytoplasm appeared in the treated cells, whereas the same concentration of 1 alpha,25-dihydroxyvitamin D3 did not affect the morphology of the parental cells. Major alterations, such as expression of type II collagen, alpha and beta chains of S-100 protein, and sulfated proteoglycans, were observed in these cells with a phenotype similar to chondrocytes. After the removal of 1 alpha,25-dihydroxyvitamin D3 from the culture, the treated cells returned rapidly to the phenotype of the untreated cells. These findings indicate that the reversible differentiation into chondrocyte-like cells of a human salivary acinar cell line occurs in growth medium containing 1 alpha,25-dihydroxyvitamin D3.
用5-氮杂胞苷处理人唾液闰管癌细胞系诱导出一种具有腺泡细胞表型的克隆细胞，将其在1α,25-二羟基维生素D3存在的情况下培养。细胞发生了形态变化；经处理的细胞中出现了大细胞，这些细胞呈多边形或圆形，细胞质中有许多空泡，而相同浓度的1α,25-二羟基维生素D3对亲代细胞的形态没有影响。在这些具有类似于软骨细胞表型的细胞中观察到了主要变化，如II型胶原蛋白、S-100蛋白的α和β链以及硫酸化蛋白聚糖的表达。从培养物中去除1α,25-二羟基维生素D3后，经处理的细胞迅速恢复到未处理细胞的表型。这些发现表明，人唾液腺泡细胞系在含有1α,25-二羟基维生素D3的生长培养基中可发生可逆性分化为软骨样细胞。
