Iga H, Azuma M, Nagamine S, Kaji R, Takase M, Yanagawa T, Yoshida H, Sato M
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
Cancer Res. 1989 Dec 1;49(23):6708-19.
A neoplastic salivary cell line with an ultrastructure similar to that of an intercalated duct cell of the salivary gland, established from a human submandibular salivary gland, has been used in our laboratory as a model for studying mechanisms regulating cytodifferentiation in salivary glands. The expression of neurofilaments (Mr 200,000, 160,000, and 68,000) in the neoplastic human salivary intercalated duct cell line and its derivatives was found by the immunofluorescence staining technique, immunoblotting, or immunoelectron microscopy. In addition, these cells stained with Bodian impregnation and expressed specific antigens such as tubulin alpha and beta chain, HNK-1 antigen, and laminin. When these cells were cultured in the presence of nerve growth factor, only the cells with a myoepithelial cell phenotype formed the long cytoplasmic processes which were densely packed with ample microfibrils in addition to microtubule bundles, and they exhibited marked suppression of anchorage-independent and anchorage-dependent growths. These findings indicate that the characteristics of neoplastic human salivary intercalated duct cell line and its derivatives are similar to those of neuronal cells.
从人下颌下唾液腺建立的一种肿瘤性唾液细胞系，其超微结构与唾液腺闰管细胞相似，在我们实验室已被用作研究唾液腺细胞分化调控机制的模型。通过免疫荧光染色技术、免疫印迹或免疫电子显微镜发现，在该肿瘤性人唾液闰管细胞系及其衍生物中存在神经丝（分子量分别为200,000、160,000和68,000）的表达。此外，这些细胞经博迪安浸染染色，并表达诸如微管蛋白α和β链、HNK - 1抗原以及层粘连蛋白等特异性抗原。当这些细胞在神经生长因子存在的情况下培养时，只有具有肌上皮细胞表型的细胞形成了长的细胞质突起，除了微管束外，这些突起还密集地排列着丰富的微原纤维，并且它们表现出对非贴壁依赖性生长和贴壁依赖性生长的显著抑制。这些发现表明，肿瘤性人唾液闰管细胞系及其衍生物的特征与神经元细胞相似。
