[中国脓肿分枝杆菌临床分离株中与氟喹诺酮耐药相关的gyrA和gyrB基因突变检测]
[Detection of mutations in the gyrA and gyrB genes associated with fluoroquinolone-resistance among clinical isolates of Mycobacterium abscessus in China].
作者信息
Chen Suting, Nie Wenjuan, Shang Yuanyuan, Liang Qian, Fu Yuhong, Ma Yifeng, Chu Naihui, Huang Hairong
机构信息
National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-resistant Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-resistant Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China; Email:
出版信息
Zhonghua Jie He He Hu Xi Za Zhi. 2015 Jul;38(7):507-10.
OBJECTIVE
To determine molecular mutations in gyrA and gyrB genes and their association with fluoroquinone-resistance among Mycobacterium abscessus isolates from China.
METHODS
Antimicrobial susceptibility profile of Mycobacterium abscessus isolates was evaluated for resistance to levofloxacin (LFX) and moxifloxacin (MFX). The quinolone resistant determing regions (QRDR) of gyrA and gyrB genes of all the isolates was sequenced and analyzed.
RESULTS
A total 70 Mycobacterium abscessus isolates were analyzed, including 45 (64%) M. abscessus subsp. abscessus and 25 (36%) M. abscessus subsp. massiliense. Analysis of antimicrobial susceptibility profile showed 97% (n=68) of isolates were resistance to LFX and 14.3% (n=10) of isolates were resistance to MFX. All the MFX-resistant strains were resistant to LFX. Cross-resistance to LFX and MFX was noted in 14.3% (n=10) of isolates. Sequencing analysis revealed that the Ser83Ala substitution in the gyrA gene and Lys447Arg and Ser464Asn substitutions in the gyrB gene were found in all the isolates from both M. abscessus subsp. abscessus and M. abscessus subsp. massiliense. No mutation was found to be associated with cross-resistance of M. abscessus to LFX and MFX in the entire gyrA and gyrB gene.
CONCLUSIONS
Our data suggest that there is no clear correlation between the type of mutation in the gene gyrA and gyrB, and the MIC levels of LFX and MFX for resistant M. abscessus strains.
目的
确定中国脓肿分枝杆菌分离株中gyrA和gyrB基因的分子突变及其与氟喹诺酮耐药性的关系。
方法
评估脓肿分枝杆菌分离株对左氧氟沙星（LFX）和莫西沙星（MFX）的抗菌药敏谱。对所有分离株的gyrA和gyrB基因的喹诺酮耐药决定区（QRDR）进行测序和分析。
结果
共分析了70株脓肿分枝杆菌分离株，其中包括45株（64%）脓肿分枝杆菌脓肿亚种和25株（36%）脓肿分枝杆菌马赛亚种。抗菌药敏谱分析显示，97%（n=68）的分离株对LFX耐药，14.3%（n=10）的分离株对MFX耐药。所有对MFX耐药的菌株均对LFX耐药。14.3%（n=10）的分离株中发现对LFX和MFX有交叉耐药性。测序分析显示，在脓肿分枝杆菌脓肿亚种和脓肿分枝杆菌马赛亚种的所有分离株中均发现gyrA基因中的Ser83Ala替换以及gyrB基因中的Lys447Arg和Ser464Asn替换。在整个gyrA和gyrB基因中未发现与脓肿分枝杆菌对LFX和MFX交叉耐药相关的突变。
结论
我们的数据表明，gyrA和gyrB基因的突变类型与耐药脓肿分枝杆菌菌株的LFX和MFX MIC水平之间没有明显的相关性。
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