Unger E R, Brigati D J
Curr Top Microbiol Immunol. 1989;143:21-31. doi: 10.1007/978-3-642-74425-9_3.
Automation of in situ hybridization is an important first step toward practical implementation of the widely recognized diagnostic potential of nucleic acid hybridization. Our laboratory has concentrated its efforts towards automating colorimetric in situ hybridization on formalin-fixed paraffin-embedded tissue sections. We have capitalized upon the technology developed for the automation of immunohistochemistry (Brigati et al. 1988) and are collaborating with Fisher Scientific in modifying the Fisher Code-On Stainer to achieve successful automated in situ hybridization. Preliminary results are encouraging. We feel that the capillary gap technology has the potential to be modified to automate other hybridization assay formats such as dot and sandwich blot hybridizations. While specifically developed for colorimetric hybridization, the instrumentation is self-contained and could be safely adapted to the use of radio-labeled probes if necessary.
原位杂交自动化是朝着实际应用核酸杂交被广泛认可的诊断潜力迈出的重要第一步。我们实验室一直致力于实现福尔马林固定石蜡包埋组织切片上比色原位杂交的自动化。我们利用了为免疫组织化学自动化开发的技术（Brigati等人，1988年），并正在与Fisher Scientific合作，对Fisher Code-On染色机进行改造，以实现成功的自动化原位杂交。初步结果令人鼓舞。我们认为，毛细管间隙技术有潜力进行改造，以实现其他杂交检测形式的自动化，如斑点杂交和夹心印迹杂交。虽然该仪器是专门为比色杂交开发的，但它是独立的，如果有必要，可以安全地适用于放射性标记探针的使用。
