Khochbin S, Chabanas A, Albert P, Lawrence J J
Departement de Recherche Fondamentale, Unité Inserm 309, CEN-Grenoble, France.
Cytometry. 1989 Jul;10(4):484-9. doi: 10.1002/cyto.990100418.
Quantitative treatment of multiparametric determination on cells using flow cytofluorometry was made possible by the development of a computer program that allows the relative quantification of a specific protein as a function of the position of cells during the cell cycle. This type of analysis provides interesting information about the distribution of a given protein throughout the cell cycle. Four examples showing the distribution of specific proteins illustrate such a quantification during the cell cycle in two different cell lines. The program also allows for the handling of a series of histograms obtained by the analysis of protein distribution as a function of DNA content in relation to a third parameter. To illustrate possible applications for this program, the evolution of the distribution of two proteins, the oncoprotein p53 and the histone H1(0), during the induced differentiation of murine erythroleukemia cells has been studied.
通过开发一种计算机程序，利用流式细胞荧光术对细胞进行多参数测定的定量处理成为可能。该程序可根据细胞在细胞周期中的位置，对特定蛋白质进行相对定量。这种分析类型提供了有关给定蛋白质在整个细胞周期中分布的有趣信息。四个展示特定蛋白质分布的例子说明了在两种不同细胞系的细胞周期中的这种定量情况。该程序还允许处理通过分析蛋白质分布与DNA含量相关的第三个参数而获得的一系列直方图。为了说明该程序的可能应用，研究了在小鼠红白血病细胞诱导分化过程中两种蛋白质，即癌蛋白p53和组蛋白H1(0)的分布演变。
