Bacteriology-Virology laboratory, CHU A. le Dantec, Cheikh Anta Diop University of Dakar, Dakar, Senegal.
Regional center for research and training on HIV/AIDS CHU Fann, Dakar, Senegal.
J Med Virol. 2017 Mar;89(3):484-488. doi: 10.1002/jmv.24460. Epub 2016 Nov 9.
The aim of this pilot study was to analyze the Hepatitis C Virus (HCV) genotypes circulating in Senegal among Drug User (DUs), using Dried Blood Spots (DBS) as RNA source for molecular assays. Heroin and/or cocaine users (n = 506) were recruited in Dakar from April to July 2011, using a Respondent Driven Sampling (RDS) method. DBS preparation consisted of five drops of whole blood from finger applied to a Whatman paper card. HCV infection was screened by the detection of anti-HCV antibodies, using a rapid immune-chromatographic test. HCV RNA was quantified on anti-HCV positive DBS, using the Abbott RealTime HCV® Genotyping was performed on DBS with detectable viral load with Versant® HCV Genotype 2.0 Assay (LiPA) and Abbott RealTime HCV Genotype II assay®. Among the 506 participants, 120 were tested as positive for anti-HCV antibodies and their samples were analyzed for HCV RNA viral load and genotype. Out of the 120 DBS tested, HCV RNA was detected on 25 (20.8%). The median viral load was 15,058 IU/ml (ranging from 710 to 766,740 IU/ml). All positive DBS were suitable for the genotyping assay, that showed a predominance of genotype 1 (21/25) including 16 genotypes 1a and 5 genotypes 1b. HCV genotype 1 prevails in a DU population in Dakar. DBS could be useful for HCV RNA genotyping, but optimal storage conditions should required avoiding RNA impairment. Acknowledging this limitation, DBS could be a great interest for detecting and genotyping HCV viremic patients. J. Med. Virol. 89:484-488, 2017. © 2015 Wiley Periodicals, Inc.
本初步研究旨在利用 DBS 作为分子检测的 RNA 来源，分析塞内加尔吸毒者中流行的丙型肝炎病毒 (HCV) 基因型。2011 年 4 月至 7 月，采用回应者驱动抽样（RDS）方法，在达喀尔招募了海洛因和/或可卡因使用者（n = 506）。DBS 制备包括将来自手指的五滴血滴加至 Whatman 滤纸卡上。使用快速免疫层析试验检测抗 HCV 抗体，对 HCV 感染进行筛查。在抗 HCV 阳性 DBS 上定量 HCV RNA，使用 Abbott RealTime HCV® Genotyping 检测试剂盒（LiPA）和 Abbott RealTime HCV Genotype II assay®对具有可检测病毒载量的 DBS 进行 HCV 基因型检测。在 506 名参与者中，有 120 名被检测为抗 HCV 抗体阳性，对其样本进行 HCV RNA 病毒载量和基因型分析。在检测的 120 个 DBS 中，有 25 个（20.8%）检测到 HCV RNA。中位病毒载量为 15,058 IU/ml（范围为 710 至 766,740 IU/ml）。所有阳性 DBS 均适合进行基因分型检测，结果显示以基因型 1 为主（21/25），包括 16 种基因型 1a 和 5 种基因型 1b。HCV 基因型 1 在达喀尔的吸毒者中流行。DBS 可用于 HCV RNA 基因分型，但需要避免 RNA 损伤，应优化储存条件。鉴于此限制，DBS 可能对检测和基因分型 HCV 病毒血症患者具有重要意义。J. Med. Virol. 89:484-488, 2017. © 2015 Wiley Periodicals, Inc.
