Koscsó Balázs, Gowda Kavitha, Bogunovic Milena
Department of Microbiology and Immunology, Penn State University College of Medicine and Milton Hershey Medical Center, Hershey, PA 17033, USA.
Department of Microbiology and Immunology, Penn State University College of Medicine and Milton Hershey Medical Center, Hershey, PA 17033, USA.
J Immunol Methods. 2016 May;432:13-23. doi: 10.1016/j.jim.2015.12.009. Epub 2015 Dec 17.
Mononuclear phagocytes (MPs) are an essential component of the intestinal immune system. They are comprised of a few dendritic cell and macrophage subsets, all with the common ability to sample extracellular milieu and to discriminate between dangerous and innocuous signals. Despite the commonality, each MP subset acquires distinct developmental pathways and unique functions, likely to fulfill needs of the tissue in which they reside. Some MP subsets develop from monocytes and are distinguished by their expression of CX3C-chemokine receptor 1 (CX3CR1). This manuscript summarizes our expertise in vivo targeting of intestinal CX3CR1(+) MP subsets. The described tools might be useful for studies of CX3CR1(+) MP function in various murine experimental models, particularly under non-inflammatory conditions.
单核吞噬细胞（MPs）是肠道免疫系统的重要组成部分。它们由一些树突状细胞和巨噬细胞亚群组成，所有这些亚群都具有共同的能力来采样细胞外环境并区分危险信号和无害信号。尽管存在共性，但每个MP亚群都有不同的发育途径和独特的功能，这可能是为了满足它们所驻留组织的需求。一些MP亚群由单核细胞发育而来，并以其CX3C趋化因子受体1（CX3CR1）的表达为特征。本手稿总结了我们在体内靶向肠道CX3CR1(+) MP亚群方面的专业知识。所描述的工具可能有助于在各种小鼠实验模型中研究CX3CR1(+) MP的功能，特别是在非炎症条件下。
