Boros Ákos, Pankovics Péter, Adonyi Ádám, Fenyvesi Hajnalka, Day J Michael, Phan Tung Gia, Delwart Eric, Reuter Gábor
Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary and University of Pécs, Pécs, Hungary.
United States Department of Agriculture, Agricultural Research Service, U. S. National Poultry Research Center, Southeast Poultry Research Laboratory, Athens, GA, USA.
Virology. 2016 Feb;489:63-74. doi: 10.1016/j.virol.2015.12.002. Epub 2015 Dec 18.
In this study all currently known chicken picornaviruses including a novel one (chicken phacovirus 1, KT880670) were identified by viral metagenomic and RT-PCR methods from a single specimen of a diarrheic chicken suffering from a total of eight picornavirus co-infections, in Hungary. The complete genomes of six picornaviruses were determined and their genomic and phylogenetic characteristics and UTR RNA structural models analyzed in details. Picornaviruses belonged to genera Sicinivirus (the first complete genome), Gallivirus, Tremovirus, Avisivirus and "Orivirus" (two potential genotypes). In addition, the unassigned phacoviruses were also detected in multiple samples of chickens in the USA. Multiple co-infections promote and facilitate the recombination and evolution of picornaviruses and eventually could contribute to the severity of the diarrhea in chicken, in one of the most important food sources of humans.
在本研究中，通过病毒宏基因组学和RT-PCR方法，从匈牙利一只腹泻鸡的单个样本中鉴定出了所有目前已知的鸡微小RNA病毒，包括一种新型病毒（鸡噬病毒1，KT880670），该样本总共感染了8种微小RNA病毒。测定了6种微小RNA病毒的完整基因组，并详细分析了它们的基因组和系统发育特征以及UTR RNA结构模型。微小RNA病毒属于Sicinivirus属（首个完整基因组）、鸡病毒属、震颤病毒属、禽病毒属和“正痘病毒属”（两种潜在基因型）。此外，在美国鸡的多个样本中也检测到了未分类的噬病毒。多重共感染促进并推动了微小RNA病毒的重组和进化，最终可能导致鸡腹泻症状加重，而鸡是人类最重要的食物来源之一。
