Liu Mi-Hua, Lin Xiao-Long, Li Jian, He Jun, Tan Tian-Ping, Wu Shao-Jian, Yu Shan, Chen Li, Liu Jun, Tian Wei, Chen Yu-Dan, Fu Hong-Yun, Yuan Cong, Zhang Yuan
Department of Clinical Laboratory, Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
Department of Pathology, The Third People's Hospital of Huizhou Affiliated to Guangzhou Medical University, Huizhou, Guangdong 516002, P.R. China.
Mol Med Rep. 2016 Feb;13(2):1689-94. doi: 10.3892/mmr.2015.4695. Epub 2015 Dec 18.
Resveratrol is a polyphenolic compound found in wine, which is mainly produced by the grapevine and exerts chemopreventive effects against hepatocellular carcinoma. However, the underlying molecular mechanisms have remained to be fully elucidated. The present study assessed whether resveratrol-induced apoptosis was mediated via the activation of the forkhead box O3a (FoxO3a) transcription factor. It was demonstrated that resveratrol treatment induced apoptosis in HepG2 cells, and that this pro-apoptotic effect was accompanied with increases in the expression of apoptotic protein Bim. Following resveratrol treatment, Akt-mediated phosphorylation of FoxO3a was observed to be diminished in HepG2 cells. Furthermore, resveratrol enhanced the nuclear levels of FoxO3a and mediated neuronal death via Bim. The present study demonstrated that resveratrol induced apoptosis in HepG2 cells through activation of the transcription factor FoxO3a and increasing the expression of Bim protein.
白藜芦醇是一种存在于葡萄酒中的多酚类化合物，主要由葡萄藤产生，对肝细胞癌具有化学预防作用。然而，其潜在的分子机制仍有待充分阐明。本研究评估了白藜芦醇诱导的细胞凋亡是否通过叉头框O3a（FoxO3a）转录因子的激活介导。结果表明，白藜芦醇处理可诱导HepG2细胞凋亡，且这种促凋亡作用伴随着凋亡蛋白Bim表达的增加。白藜芦醇处理后，观察到HepG2细胞中Akt介导的FoxO3a磷酸化减少。此外，白藜芦醇提高了FoxO3a的核水平，并通过Bim介导神经元死亡。本研究表明，白藜芦醇通过激活转录因子FoxO3a和增加Bim蛋白的表达诱导HepG2细胞凋亡。
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