Van Tassell Maxwell L, Angela Daum M, Kim Jun-Seob, Miller Michael J
Department of Food Science & Human Nutrition, University of Illinois, Urbana, IL 61801, USA.
Department of Food Science & Human Nutrition, University of Illinois, Urbana, IL 61801, USA.
Curr Opin Biotechnol. 2016 Feb;37:88-96. doi: 10.1016/j.copbio.2015.10.006. Epub 2015 Dec 19.
Cell wall lytic enzymes have been of increasing interest as antimicrobials for targeting Gram-positive spoilage and pathogenic bacteria, largely due to the development of strains resistant to antibiotics and bacteriophage therapy. Such lysins show considerable promise against Listeria monocytogenes, a primary concern in food-processing environments, but there is room for improvement via protein engineering. Advances in antilisterial applications could benefit from recent developments in lysin biotechnology that have largely targeted other organisms. Herein we present various considerations for the future development of lysins, including environmental factors, cell physiology concerns, and dynamics of protein architecture. Our goal is to review key developments in lysin biotechnology to provide a contextual framework for the current models of lysin-cell interactions and highlight key considerations for the characterization and design of novel lytic enzymes.
细胞壁裂解酶作为针对革兰氏阳性腐败菌和病原菌的抗菌剂越来越受到关注，这主要是由于对抗生素和噬菌体疗法产生抗性的菌株不断出现。这类溶素对食品加工环境中的主要关注点——单核细胞增生李斯特菌显示出相当大的应用前景，但通过蛋白质工程仍有改进空间。抗李斯特菌应用的进展可受益于溶素生物技术的最新发展，这些发展主要针对其他生物体。在此，我们提出了溶素未来发展的各种考虑因素，包括环境因素、细胞生理学问题和蛋白质结构动态。我们的目标是回顾溶素生物技术的关键发展，为当前溶素-细胞相互作用模型提供一个背景框架，并突出新型裂解酶表征和设计的关键考虑因素。
