Zhang Shanshan, Li Jiaojiao, Zhang Fang, Zhang Xiaofeng, Lu Shenzhou
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2015 Aug;32(4):867-73, 886.
It is important to design a long-period transparent bioactive material for corneal repair in the process of corneal tissue renovation. This article discusses the silk fibroin and formamide blend membranes as a corneal stroma repair material. Silk fibroin solution was mixed with formamide in different proportions to obtain insoluble transparent silk fibroin film by casting method. The blending membranes had excellent mechanical properties, cell compatibility and long-term transparent properties. Rabbit corneal stromal cells were seeded on the sterilized composite films. The rate of cell surface adhesion was over 90% after cells were placed on it for 5 hours. When cells were seeded on blend membranes from one day to seven days, Alma Blue was added to complete medium. Compared with the cell culture plate, there was no significant difference in cell proliferation on formamide/silk films. The results indicated that formamide/silk films might be used as a corneal stroma repair material and worth of further investigatinn
在角膜组织修复过程中，设计一种用于角膜修复的长期透明生物活性材料非常重要。本文讨论了丝素蛋白与甲酰胺共混膜作为角膜基质修复材料。将丝素蛋白溶液与不同比例的甲酰胺混合，通过流延法获得不溶性透明丝素蛋白膜。该共混膜具有优异的力学性能、细胞相容性和长期透明性能。将兔角膜基质细胞接种于灭菌后的复合膜上。细胞接种5小时后，细胞表面黏附率超过90%。当细胞接种于共混膜上1天至7天时，在完全培养基中加入阿尔玛蓝。与细胞培养板相比，甲酰胺/丝素蛋白膜上细胞增殖无显著差异。结果表明，甲酰胺/丝素蛋白膜可作为角膜基质修复材料，值得进一步研究。
