Hazra Sarbani, Nandi Sudip, Naskar Deboki, Guha Rajdeep, Chowdhury Sushovan, Pradhan Nirparaj, Kundu Subhas C, Konar Aditya
Department of Veterinary Surgery &Radiology, West Bengal University of Animal &Fishery Sciences, Kolkata-700037, West Bengal, India.
CSIR-Indian Institute of Chemical Biology, Kolkata-700032, West Bengal, India.
Sci Rep. 2016 Feb 24;6:21840. doi: 10.1038/srep21840.
Successful repair of a damaged corneal surface is a great challenge and may require the use of a scaffold that supports cell growth and differentiation. Amniotic membrane is currently used for this purpose, in spite of its limitations. A thin transparent silk fibroin film from non-mulberry Antheraea mylitta (Am) has been developed which offers to be a promising alternative. The silk scaffolds provide sufficient rigidity for easy handling, the scaffolds support the sprouting, migration, attachment and growth of epithelial cells and keratocytes from rat corneal explants; the cells form a cell sheet, preserve their phenotypes, express cytokeratin3 and vimentin respectively. The films also support growth of limbal stem cell evidenced by expression of ABCG2. The cell growth on the silk film and the amniotic membrane is comparable. The implanted film within the rabbit cornea remains transparent, stable. The clinical examination as well as histology shows absence of any inflammatory response or neovascularization. The corneal surface integrity is maintained; tear formation, intraocular pressure and electroretinography of implanted eyes show no adverse changes. The silk fibroin film from non-mulberry silk worms may be a worthy candidate for use as a corneal scaffold.
成功修复受损的角膜表面是一项巨大挑战，可能需要使用支持细胞生长和分化的支架。尽管羊膜有其局限性，但目前仍用于此目的。已开发出一种来自非桑蚕丝的薄透明丝素蛋白膜（Am），它有望成为一种替代物。丝素蛋白支架具有足够的刚性便于操作，该支架支持大鼠角膜外植体上皮细胞和角膜细胞的发芽、迁移、附着和生长；细胞形成细胞片，保持其表型，分别表达细胞角蛋白3和波形蛋白。这些膜还通过ABCG2的表达证明支持角膜缘干细胞的生长。丝素蛋白膜和羊膜上的细胞生长情况相当。植入兔角膜内的膜保持透明、稳定。临床检查和组织学检查均显示无任何炎症反应或新生血管形成。角膜表面完整性得以维持；植入眼的泪液形成、眼压和视网膜电图均未显示出不利变化。非桑蚕丝的丝素蛋白膜可能是用作角膜支架的一个有价值的候选物。
