红细胞葡萄糖-6-磷酸脱氢酶的年龄依赖性检测及其与氧化应激的相关性。
Age-dependent detection of erythrocytes glucose-6-phosphate dehydrogenase and its correlation with oxidative stress.
作者信息
Maurya Pawan Kumar, Kumar Prabhanshu, Chandra Pranjal
机构信息
a Interdisciplinary Laboratory for Clinical Neuroscience (LiNC) , Department of Psychiatry, Universidade Federal de Sao Paulo - UNIFESP , Sao Paulo , Brazil .
b Amity Institute of Biotechnology , Amity University Uttar Pradesh , Noida, Uttar Pradesh , India , and.
出版信息
Arch Physiol Biochem. 2016;122(2):61-6. doi: 10.3109/13813455.2015.1136648. Epub 2016 Jan 27.
CONTEXT
Glucose-6-phosphate dehydrogenase (G6PD) is an important enzyme of hexose monophosphate shunt, involved in the biosynthesis of reduced nicotinamide adenine dinucleotide phosphate hydrogen (NADPH).
OBJECTIVE
This study was designed to investigate age-dependent changes in human erythrocyte G6PD activity. The G6PD activity pattern was correlated with reduced glutathione (GSH) and total antioxidant potential in terms of FRAP (ferric reducing ability of plasma) value.
MATERIALS AND METHODS
We analyzed normal, healthy subjects of both sexes between the ages of 20 and 80 years. G6PD activity was determined by Burties method.
RESULTS
We observe a significant age-dependent decrease in G6PD activity (p < 0.0001). It was positively correlated with GSH (r = 0.5706) and total antioxidant potential (r = 0.7723) as a function of human age.
DISCUSSION AND CONCLUSION
Our findings on erythrocyte G6PD and their correlation with GSH and FRAP provide evidence of a higher oxidative stress in old age population.
背景
葡萄糖-6-磷酸脱氢酶（G6PD）是磷酸己糖旁路的一种重要酶，参与还原型烟酰胺腺嘌呤二核苷酸磷酸氢（NADPH）的生物合成。
目的
本研究旨在调查人类红细胞G6PD活性的年龄依赖性变化。G6PD活性模式与还原型谷胱甘肽（GSH）以及基于血浆铁还原能力（FRAP）值的总抗氧化能力相关。
材料与方法
我们分析了年龄在20至80岁之间的正常健康男女受试者。G6PD活性通过伯蒂斯方法测定。
结果
我们观察到G6PD活性存在显著的年龄依赖性下降（p < 0.0001）。它与GSH（r = 0.5706）以及作为人类年龄函数的总抗氧化能力（r = 0.7723）呈正相关。
讨论与结论
我们关于红细胞G6PD及其与GSH和FRAP相关性的研究结果为老年人群中更高的氧化应激提供了证据。
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