Volod'ko A V, Davydova V N, Glazunov V P, Likhatskaya G N, Yermak I M
G.B Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia.
G.B Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia.
Int J Biol Macromol. 2016 Mar;84:434-41. doi: 10.1016/j.ijbiomac.2015.12.031. Epub 2015 Dec 19.
The polyelectrolyte complexes (PEC) of carrageenans (CG)-κ-, κ/β-, λ-and x-CG with chitosan were obtained. The formation of PEC was detected by Fourier-transform infrared (FTIR) spectroscopy and by centrifugation in a Percoll gradient. The influence of the structural peculiarities of CG on its interaction with chitosan was studied. The results of centrifugation showed that x-CG with a high degree of sulphation (SD) was completely bound to chitosan, unlike low SD κ-CG and κ/β-CG. Binding constant values showed there was a high affinity of CG for chitosan. CG with flexible macromolecule conformation and high SD exhibited the greatest binding affinity for chitosan. The full-atomic 3D-structures of the PEC κ-CG: chitosan in solution have been obtained by the experiments in silico for the first time. The amino groups of chitosan make the largest contribution to the energy of the complex formation by means of hydrogen and ionic bonds. The most probable complexes have stoichiometries of 1:1 and 1:1.5.
获得了角叉菜胶（CG）（κ-、κ/β-、λ-和x-CG）与壳聚糖的聚电解质复合物（PEC）。通过傅里叶变换红外（FTIR）光谱和在Percoll梯度中离心检测PEC的形成。研究了CG结构特性对其与壳聚糖相互作用的影响。离心结果表明，与低硫酸化度（SD）的κ-CG和κ/β-CG不同，高SD的x-CG与壳聚糖完全结合。结合常数表明CG对壳聚糖具有高亲和力。具有柔性大分子构象和高SD的CG对壳聚糖表现出最大的结合亲和力。首次通过计算机模拟实验获得了溶液中PEC κ-CG:壳聚糖的全原子三维结构。壳聚糖的氨基通过氢键和离子键对复合物形成的能量贡献最大。最可能的复合物化学计量比为1:1和1:1.5。
