Broderick G A, Tom R, McClure R D
Department of Urology, University of California, San Francisco 94143-0738.
J Urol. 1989 Sep;142(3):752-5. doi: 10.1016/s0022-5347(17)38877-8.
With the immunobead antisperm antibody test a prospective study was conducted to evaluate the immune status of 55 men before and after vasectomy reversal. A third of the vasectomy patients (19 of 55) had significant serum-sperm antibodies (20 per cent binding or more) detected by the indirect immunobead antisperm antibody test. Of 31 vasovasostomy patients 12 (38 per cent) had significant sperm-surface antibodies (20 per cent binding or more) by the direct immunobead antisperm antibody test. Preoperative serum assays correctly classified the antibody status of 69 per cent of the vasectomy patients. The immunological impact of spermatic granuloma formation, duration of vasal obstruction, patient age and presence of sperm in the vasal fluid at operation also were assessed. An inverse relationship between the proportion of antibody-bound sperm and the percentage of motile sperm in the ejaculate of vas reversal patients was found with videomicrographic semen analysis. The percentage motility was significantly lower among patients with greater quantities of sperm-surface antibodies. No other parameter of semen analysis showed this difference when compared for positive or negative immunobead antisperm antibody test results.
通过免疫珠抗精子抗体检测，进行了一项前瞻性研究，以评估55名男性在输精管复通术前和术后的免疫状态。在输精管结扎患者中，三分之一（55名中的19名）通过间接免疫珠抗精子抗体检测发现有显著的血清精子抗体（结合率达20%或更高）。在31名输精管吻合术患者中，12名（38%）通过直接免疫珠抗精子抗体检测发现有显著的精子表面抗体（结合率达20%或更高）。术前血清检测正确分类了69%输精管结扎患者的抗体状态。还评估了精索肉芽肿形成的免疫影响、输精管梗阻的持续时间、患者年龄以及手术时输精管液中精子的存在情况。通过视频显微镜精液分析发现，输精管复通患者射精中抗体结合精子的比例与活动精子的百分比之间呈负相关。精子表面抗体量较多的患者，其活动率显著较低。当比较免疫珠抗精子抗体检测结果为阳性或阴性时，精液分析的其他参数均未显示出这种差异。
