Yoshioka K, Takemura T, Tohda M, Akano N, Miyamoto H, Ooshima A, Maki S
Department of Pediatrics, Kinki University School of Medicine, Osaka, Japan.
Kidney Int. 1989 May;35(5):1203-11. doi: 10.1038/ki.1989.111.
Kidney specimens taken from normal humans and patients with various renal diseases were examined by immunofluorescent and immunoelectron microscopy using a monoclonal antibody to the alpha 1 chain of type III collagen. Indirect immunofluorescent staining revealed that intraglomerular localization of type III collagen antigen in 41 of 66 patients, while it was absent from the glomeruli of normal human kidneys. Type III collagen was found within the mesangium of 22 patients with various types of renal diseases, and was distributed in a focal and segmental manner in most of the cases. Mesangial localization of the collagen correlated with the increase in the mesangial matrix. Type III collagen was also present in the vascular pole, crescents (particularly in the organizing phase) and sclerosed glomeruli. Immunoelectron microscopy using pre-embedding and post-embedding techniques confirmed the above observations. These findings indicate that type III collagen participates in mesangial expansion, crescent organization, and glomerulosclerosis.
使用针对III型胶原α1链的单克隆抗体，通过免疫荧光和免疫电子显微镜对取自正常人和患有各种肾脏疾病患者的肾脏标本进行了检查。间接免疫荧光染色显示，66例患者中有41例肾小球内存在III型胶原抗原，而正常人类肾脏的肾小球中不存在该抗原。在22例患有各种类型肾脏疾病的患者的系膜中发现了III型胶原，并且在大多数情况下呈局灶性和节段性分布。胶原的系膜定位与系膜基质的增加相关。III型胶原也存在于血管极、新月体（特别是在组织期）和硬化的肾小球中。使用包埋前和包埋后技术的免疫电子显微镜证实了上述观察结果。这些发现表明III型胶原参与了系膜扩张、新月体组织形成和肾小球硬化。
