夹心酶联免疫吸附测定法检测脑脊液中HIV-Tat蛋白的方案
Protocol for Detection of HIV-Tat Protein in Cerebrospinal Fluid by a Sandwich Enzyme-Linked Immunosorbent Assay.
作者信息
Johnson Tory P, Nath Avindra
机构信息
Section of Infections of the Nervous System, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Room 7C-103, Bldg 10; 10 Center Drive, Bethesda, MD, 20892, USA.
出版信息
Methods Mol Biol. 2016;1354:343-52. doi: 10.1007/978-1-4939-3046-3_23.
Abstract
The human immunodeficiency virus (HIV) transactivator of transcription (Tat) is a virally produced protein that is required for efficient viral replication. Once formed inside an infected cell, Tat is secreted into the extracellular space where it has pathophysiological consequences on cells it interacts with. Tat has been demonstrated to be neurotoxic and is produced even under the pressures of anti-retroviral therapy; therefore Tat is suspected to contribute to the development of HIV-associated neurocognitive disorders. In this chapter, we describe a sandwich enzyme-linked immunosorbent assay protocol for the detection of Tat from cerebrospinal fluid samples.
摘要
人类免疫缺陷病毒（HIV）转录反式激活因子（Tat）是一种病毒产生的蛋白质，是高效病毒复制所必需的。一旦在受感染细胞内形成，Tat就会分泌到细胞外空间，在那里它会对与其相互作用的细胞产生病理生理影响。Tat已被证明具有神经毒性，即使在抗逆转录病毒治疗的压力下也会产生；因此，怀疑Tat会导致与HIV相关的神经认知障碍的发展。在本章中，我们描述了一种用于检测脑脊液样本中Tat的夹心酶联免疫吸附测定方法。
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