Li Wanyun, Zhang Na, Ou Yurong, Zhou Zhengguang, Zhao Fuyou, Wu Qiong, Yang Yan
Department of Pathology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Dec;35(12):1705-9, 1720.
To investigate the role of triggering receptors expressed on myeloid cells-1 (TREM-1) in the oncogenesis and progression of hepatocellular carcinoma (HCC).
The expression and localization of TREM-1 were detected by immunohistochemistry in 76 specimens of HCC, 33 specimens of liver cirrhosis, 30 specimens of hepatitis and 20 normal liver tissues. The association between TREM-1 expression and the clinicopathologic parameters of HCC was analyzed. Human normal hepatic cell line LO2 and HCC cell line SMMC-7721 were examined for TREM-1 expression pattern using RT-PCR and Western blotting.
All the normal liver samples showed negative expression of TREM-1 protein, which was significantly up-regulated in the other 3 tissues. The positivity rates of TREM-1 expression were not significantly different between hepatitis, cirrhosis and HCC tissues [20.00% (6/30), 24.24% (8/33), and 21.05% (16/76), respectively; Χ² =0.195, P=0.907]. Different from chronic hepatitis and liver cirrhosis tissues where TREM-1 expression was located mainly in the nucleus and occasionally in the cytoplasm of the hepatocytes, HCC tissues showed a cellular localization of TREM-1 protein almost exclusively in the cytoplasm. In HCC, TREM-1 expression was negatively correlated with the histological grade of the tumor (r=-0.261, P=0.023) but not related with the patients' age, gender, tumor size, clinical stage, pre-existing hepatitis and cirrhosis, lymph node metastasis, or intrahepatic vascular embolism (all P>0.05). In the in vitro experiments, low levels of TREM-1 mRNA and protein expressions were detected in LO2 cells line, but their expressions were markedly up-regulated in SMMC-7721 cells.
Aberrant enhancement of the expression and cytoplasmic accumulation of TREM-1 may correlate closely with the oncogenesis and progression of HCC.
探讨髓系细胞触发受体-1（TREM-1）在肝细胞癌（HCC）发生发展中的作用。
采用免疫组织化学法检测76例HCC标本、33例肝硬化标本、30例肝炎标本及20例正常肝组织中TREM-1的表达及定位。分析TREM-1表达与HCC临床病理参数的相关性。采用RT-PCR和Western印迹法检测人正常肝细胞系LO2和HCC细胞系SMMC-7721中TREM-1的表达模式。
所有正常肝组织样本TREM-1蛋白均呈阴性表达，在其他3种组织中显著上调。肝炎、肝硬化和HCC组织中TREM-1表达的阳性率差异无统计学意义[分别为20.00%（6/30）、24.24%（8/33）和21.05%（16/76）；χ² =0.195，P=0.907]。与慢性肝炎和肝硬化组织中TREM-1表达主要位于细胞核且偶尔位于肝细胞胞质不同，HCC组织中TREM-1蛋白几乎完全定位于细胞质。在HCC中，TREM-1表达与肿瘤组织学分级呈负相关（r=-0.261，P=0.023），但与患者年龄、性别、肿瘤大小、临床分期、既往肝炎和肝硬化、淋巴结转移或肝内血管栓塞均无关（均P>0.05）。体外实验中，LO2细胞系中检测到低水平的TREM-1 mRNA和蛋白表达，但在SMMC-7721细胞中其表达明显上调。
TREM-1表达的异常增强及细胞质积聚可能与HCC的发生发展密切相关。
