Chan J, Babb R, David S C, McColl S R, Alsharifi M
Vaccine Research Group, Centre for Molecular Pathology, School of Biological Sciences, The University of Adelaide, Adelaide, SA, Australia.
Scand J Immunol. 2016 Mar;83(3):165-73. doi: 10.1111/sji.12410.
During acute viral infections, innate immunity provides essential protective measures to minimize virus dissemination and regulate adaptive immunity. This helps to successfully eliminate the pathogen and establish long-term memory. Here, we investigated the effect of vaccine-induced antibody responses on the induction of IFN-I responses and the associated lymphocyte activation using influenza A virus vaccination and challenge models. Mice were vaccinated with gamma-irradiated influenza A virus (γ-FLU) and challenged three weeks later with live virus. Our data show a significant reduction in IFN-I responses and lymphocyte activation following a homotypic virus challenge. We confirmed the role of vaccine-induced antibody responses in the observed impairment of IFN-I and the associated lymphocyte activation using adoptive transfer of immune sera and the administration of sera-treated viruses prior to challenge. Overall, we addressed a fundamental concept in immunology and provided experimental data illustrating the inhibition of IFN-I responses in vaccinated animals upon a homotypic virus challenge.
在急性病毒感染期间，先天免疫提供了重要的保护措施，以尽量减少病毒传播并调节适应性免疫。这有助于成功清除病原体并建立长期记忆。在此，我们使用甲型流感病毒疫苗接种和攻击模型，研究了疫苗诱导的抗体反应对I型干扰素（IFN-I）反应诱导及相关淋巴细胞激活的影响。用γ射线照射的甲型流感病毒（γ-FLU）对小鼠进行疫苗接种，并在三周后用活病毒进行攻击。我们的数据显示，同型病毒攻击后，IFN-I反应和淋巴细胞激活显著降低。我们通过免疫血清的过继转移以及在攻击前给予经血清处理的病毒，证实了疫苗诱导的抗体反应在观察到的IFN-I损伤及相关淋巴细胞激活中的作用。总体而言，我们阐述了免疫学中的一个基本概念，并提供了实验数据，说明了接种疫苗的动物在同型病毒攻击后IFN-I反应受到抑制的情况。
