Alvarado-Esquivel Cosme, Sánchez-Anguiano Luis Francisco, Hernández-Tinoco Jesús, Arreola-Cháidez Emilio, López Juan, Salcido-Meraz Karla Itzel, Estrada-Martínez Sergio, Navarrete-Flores José Antonio, Pérez-Álamos Alma Rosa, Hernández-Ochoa Marcia, Rábago-Sánchez Elizabeth, Liesenfeld Oliver
Faculty of Medicine and Nutrition, Juárez University of Durango State, Avenida Universidad S/N , 34000 Durango, Durango, Mexico.
Institute for Scientific Research "Dr. Roberto Rivera Damm", Juárez University of Durango State, Avenida Universidad S/N , 34000 Durango, Durango, Mexico.
Eur J Microbiol Immunol (Bp). 2015 Nov 4;5(4):285-92. doi: 10.1556/1886.2015.00039. eCollection 2015 Dec.
Through an age- and sex-matched case-control study, we sought to determine whether female sex workers have an increased risk of Toxoplasma gondii exposure and to determine the sociodemographic, work, clinical, and behavioral characteristics of these workers associated with T. gondii exposure. Female workers (n = 136) and controls (n = 272) were examined with enzyme-linked immunoassays (EIA) for the presence of anti-Toxoplasma IgG and IgM antibodies. IgM positive sera were additionally tested with enzyme linked-fluorescence immunoassay (ELFA). Anti-T. gondii IgG antibodies were found in 21 (15.44%) of 136 cases and in 10 (3.67%) of 272 controls (OR = 4.05; 95% CI: 1.84-8.89; P = 0.0001). Anti-T. gondii IgG levels higher than 150 IU/ml were found in 13 (9.6%) of 136 cases and in 8 (2.9%) of 272 controls (P = 0.007). Anti-T. gondii IgM antibodies were found in two cases and in six controls by EIA, but all were negative by ELFA. T. gondii seropositivity was associated with being born out of Durango State (OR = 10.47; 95% CI: 2.9-36.8; P < 0.01), injuries during sex work (OR = 6.30; 95% CI: 1.1-33.7; P = 0.03), and soil contact (OR = 4.11; 95% CI: 1.2-14.0; P = 0.02). This is the first report of an association of T. gondii infection and female sex workers.
通过一项年龄和性别匹配的病例对照研究，我们试图确定女性性工作者是否有更高的弓形虫暴露风险，并确定这些与弓形虫暴露相关的工作者的社会人口学、工作、临床和行为特征。对136名女性工作者和272名对照者进行酶联免疫吸附测定（EIA），检测抗弓形虫IgG和IgM抗体的存在情况。对IgM阳性血清另外采用酶联荧光免疫分析（ELFA）进行检测。在136例病例中有21例（15.44%）检测到抗弓形虫IgG抗体，在272名对照者中有10例（3.67%）检测到该抗体（比值比[OR]=4.05；95%置信区间[CI]：1.84-8.89；P=0.0001）。在136例病例中有13例（9.6%）抗弓形虫IgG水平高于150 IU/ml，在272名对照者中有8例（2.9%）高于此水平（P=0.007）。通过EIA在2例病例和6名对照者中检测到抗弓形虫IgM抗体，但经ELFA检测均为阴性。弓形虫血清阳性与出生于杜兰戈州以外地区（OR=10.47；95%CI：2.9-36.8；P<0.01）、性工作期间受伤（OR=6.30；95%CI：1.1-33.7；P=0.03）以及接触土壤（OR=4.11；95%CI：1.2-14.0；P=0.02）有关。这是关于弓形虫感染与女性性工作者之间关联的首次报告。
