Katsume C, Fernandes G, Iwabuchi K, Ogasawara K, Gotohda T, Good R A, Onoé K
Institute of Immunological Science, Hokkaido University.
Microbiol Immunol. 1989;33(4):313-28. doi: 10.1111/j.1348-0421.1989.tb01980.x.
Early development of T lineage cells were compared between AKR and C3H mice by using two experimental strategies--neonatal thymectomy (NTx) and bone marrow transplantation (BMT)--between these two strains of mice. After NTx, AKR mice developed less wasting disease and showed better maintenance of several T cell functions. In addition, the response of neonatal spleen cells to PHA and ConA was much greater in AKR mice than in C3H mice. Further, when AKR mice were used as recipients of BMT, cell numbers recovered from thymuses between 2 and 7 weeks after reconstitution were consistently much greater (about 10 times greater) than those from chimeras where C3H mice were used as recipients, regardless of the donor strains of bone marrow cells. However, 4 weeks after BMT the proliferative responses to ConA were consistently higher in the donor-derived thymocytes from chimeras where AKR mice were used as bone marrow donors than in those from chimeras in which C3H were donors. The present findings suggest that these differences may be attributed to characteristics of recipient microenvironment (e.g., thymic stroma) which maintain developing thymocytes and supply them to the peripheral lymphoid tissue. Alternatively the differences may to some degree also be attributable to characteristics of the thymic progenitors themselves, which may determine the rates of maturation of thymocyte functions.
通过在AKR和C3H这两种品系小鼠之间采用两种实验策略——新生期胸腺切除（NTx）和骨髓移植（BMT），比较了T细胞系细胞的早期发育情况。NTx后，AKR小鼠发生的消瘦性疾病较少，并且几种T细胞功能维持得更好。此外，新生期脾细胞对PHA和ConA的反应在AKR小鼠中比在C3H小鼠中要强得多。此外，当AKR小鼠作为BMT的受体时，重建后2至7周从胸腺中恢复的细胞数量始终比以C3H小鼠作为受体的嵌合体中的细胞数量多得多（约多10倍），而与骨髓细胞的供体品系无关。然而，BMT后4周，在以AKR小鼠作为骨髓供体的嵌合体中，供体来源的胸腺细胞对ConA的增殖反应始终高于以C3H作为供体的嵌合体中的细胞。目前的研究结果表明，这些差异可能归因于维持发育中的胸腺细胞并将其供应至外周淋巴组织的受体微环境（如胸腺基质）的特征。或者，这些差异在某种程度上也可能归因于胸腺祖细胞自身的特征，这可能决定胸腺细胞功能的成熟速率。
