Hajnal A, Falus A
National Institute of Hematology and Blood Transfusion, Daroczi, Hungary.
Microbiol Immunol. 1989;33(6):441-7. doi: 10.1111/j.1348-0421.1989.tb01993.x.
Plasmids from E. coli strains of 38 donors were transconjugated to common recipient SY663 Escherichia coli K12. The restriction patterns of the isolated plasmids were highly heterogenous. However, the streptomycin (Sm) resistance genes of the plasmids were identical or closely homologous in 29 of the 33 plasmids conferring Sm resistance. These data were based on Southern blot analysis, using the Sm resistance gene (encoding aminoglycoside phosphoryl transferase) as probe cut out from pBP1 plasmid. Our data suggest an extensive spreading of streptomycin resistance gene of this type.
来自38个供体大肠杆菌菌株的质粒被转接合到常见受体大肠杆菌K12的SY663中。分离出的质粒的限制性酶切图谱高度异质。然而，在33个赋予链霉素抗性的质粒中，有29个质粒的链霉素（Sm）抗性基因相同或密切同源。这些数据基于Southern印迹分析，使用从pBP1质粒切出的Sm抗性基因（编码氨基糖苷磷酸转移酶）作为探针。我们的数据表明这种类型的链霉素抗性基因广泛传播。
