Rozovskaia T A, Minasian Sh Kh, Kukhanova M K, Kraevskiĭ A A, Chidzhavadze Z G
Mol Biol (Mosk). 1989 Mar-Apr;23(2):449-62.
Reaction of DNA synthesis catalyzed by DNA polymerase I KF in the presence of 2'-deoxynucleoside 5'-alpha-thiotriphosphates (dNTP alpha S) was investigated. DNA with thiophosphate groups (DNA[P=S]) obtained by such a way was studied in reactions of hydrolysis and pyrophosphorolysis catalyzed by DNA polymerase I KF. It is shown that the rate of DNA elongation is decreased both on the step of incorporation of dNMP alpha S residues and on the step of incorporation of the next dNMP residue. The rate of pyrophosphorolysis of 3'-terminal dNMP alpha S was demonstrated to be one order of magnitude less in comparison with the corresponding reaction with the natural dNMP residue. Contrary, the rate of 3'----5'-exonuclease hydrolysis of both DNA[P=S] and DNA of the same structure revealed no distinguishable differences.
研究了在2'-脱氧核苷5'-α-硫代三磷酸（dNTPαS）存在下DNA聚合酶I KF催化的DNA合成反应。对通过这种方式获得的含硫代磷酸基团的DNA（DNA[P=S]）在DNA聚合酶I KF催化的水解和焦磷酸解反应中进行了研究。结果表明，在掺入dNMPαS残基的步骤以及掺入下一个dNMP残基的步骤中，DNA延伸速率均降低。与天然dNMP残基的相应反应相比，3'-末端dNMPαS的焦磷酸解速率被证明低一个数量级。相反，DNA[P=S]和相同结构的DNA的3'→5'-外切核酸酶水解速率没有明显差异。
