Department of Microbiology, Osmania University, India; Central Research Institute for Dry Land Agriculture, India.
Central Research Institute for Dry Land Agriculture, India.
Bioresour Technol. 2016 Feb;202:231-7. doi: 10.1016/j.biortech.2015.12.025. Epub 2015 Dec 14.
Two grams of delignified substrate at 10% (w/v) level was subjected to biphasic dilute acid hydrolysis using phosphoric acid, hydrochloric acid and sulfuric acid separately at 110 °C for 10 min in phase-I and 121 °C for 15 min in phase-II. Combinations of acid concentrations in two phases were varied for maximum holocellulose hydrolysis with release of fewer inhibitors, to select the suitable acid and its concentration. Among three acids, sulfuric acid in combination of 1 & 2% (v/v) hydrolyzed maximum holocellulose of 25.44±0.44% releasing 0.51±0.02 g/L of phenolics and 0.12±0.002 g/L of furans, respectively. Further, hydrolysis of delignified substrate using selected acid by varying reaction time and temperature hydrolyzed 55.58±1.78% of holocellulose releasing 2.11±0.07 g/L and 1.37±0.03 g/L of phenolics and furans, respectively at conditions of 110 °C for 45 min in phase-I & 121 °C for 60 min in phase-II.
将 2 克脱木质素底物以 10%（w/v）的浓度分别用磷酸、盐酸和硫酸在 110°C 下进行两相稀酸水解 10 分钟（相Ⅰ）和 121°C 下进行 15 分钟（相Ⅱ）。两相中酸浓度的组合变化以最大限度地水解全纤维素并释放较少的抑制剂，从而选择合适的酸及其浓度。在三种酸中，硫酸与 1%和 2%（v/v）的组合水解了 25.44±0.44%的最大全纤维素，分别释放了 0.51±0.02 g/L 的酚类和 0.12±0.002 g/L 的呋喃类物质。此外，通过改变反应时间和温度，使用选定的酸对脱木质素底物进行水解，在 110°C 下进行 45 分钟（相Ⅰ）和 121°C 下进行 60 分钟（相Ⅱ）的条件下，水解了 55.58±1.78%的全纤维素，分别释放了 2.11±0.07 g/L 和 1.37±0.03 g/L 的酚类和呋喃类物质。
