Co-culture of (VS3) and (NCIM 3498) enhances bioethanol yield from concentrated hydrolysate.

Biphasic acid hydrolysates and enzymatic hydrolysates from carbohydrate-rich , an invasive perennial deciduous shrub of semi-arid regions, were used for bioethanol production. and were used for fermentation of hexoses and pentoses. acid hydrolysate with an initial sugar concentration of 18.70 ± 0.16 g/L was concentrated to 33.59 ± 0.52 g/L by vacuum distillation. The concentrated hydrolysate was pretreated and fermented by mono- and co-culture methods either singly or in combination with enzyme hydrolysate and ethanol yields were compared. Monoculture with (VS3) and (NCIM3455) yielded maximum ethanol of 36.6 ± 1.83 g/L and 37.1 ± 1.86 g/L with a fermentation efficiency of 83.94 ± 4.20% and 84.20 ± 4.21%, respectively, after 36 h of fermentation. The ethanol yield obtained was 0.428 ± 0.02 g/g substrate and 0.429 ± 0.02 g/g substrate with a productivity of 1.017 ± 0.051 g/L/hand 1.031 ± 0.052 g/L/h, respectively. (NCIM3498) yielded maximum ethanol of 24 g/L with ethanol yield of 0.455 ± 0.02 g/g substrate and a productivity of 1.004 ± 0.050 g/L/h after 24 h of fermentation. With concentrated acid hydrolysate as substrate, (VS3) produced ethanol of 8.52 ± 0.43 g/L, whereas (NCIM3455) produced 5.96 ± 0.30 g/L of ethanol. (NCIM3498) produced 4.52 ± 0.23 g/L of ethanol by utilizing 14.66 ± 0.87 g/L of sugars. Co-culture with (VS3) addition after 18 h of addition of (NCIM3498) to the mixture of concentrated acid hydrolysate and enzyme hydrolysate produced 13.86 ± 0.47 g/L of ethanol with fermentation efficiency, ethanol yield and productivity of 87.54 ± 0.54%, 0.446 ± 2.36 g/g substrate and 0.385 ± 0.014 g/L/h, respectively. Hence, it is concluded that co-culture with and is feasible, further scaling up of fermentation of substrate for bioethanol production.