Zhang Lele, Yang Minglei, Gao Junlan, Jin Shan, Wu Zhengyan, Wu Lifang, Zhang Xin
School of Life Sciences, Anhui Agricultural University, Hefei 230036, People's Republic of China.
Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, People's Republic of China.
J Plant Physiol. 2016 Feb 1;191:36-44. doi: 10.1016/j.jplph.2015.11.014. Epub 2015 Dec 10.
Pistacia chinensis Bunge (P. chinensis) is a deciduous and dioecious perennial arbor of the family Anacardiaceae that flowers from March to April and bears fruit from September to October. There are three rapidly growing stages in the annual growth process of P. chinensis. However, the knowledge of the secondary metabolites related to P. chinensis gender and growth season remains scant. In this study, HPLC was used to qualitatively and quantitatively determine the content of the catechin hydrate, rutin, quercetin, and kaempferol contents in male and female tree inflorescences and leaves. Total phenolics and flavonoids were also detected using a spectrophotometer. The results indicated that the contents of these compounds fluctuated with seasons and they reached the highest levels in nascent leaves. The fluctuations of these compounds followed different pathways of evolution, by increasing or decreasing in male and female trees throughout the whole growth process because they had their own biological functions. Moreover, the extracts exhibited DPPH radical scavenging bioactivity and showed no significant cytotoxicity towards 3T3-L1 preadipocytes. Together, these results demonstrated that P. chinensis has great potential as an antioxidant medicine, and the best harvest time is in the spring.
黄连木是漆树科的一种落叶、雌雄异株的多年生乔木，花期为3月至4月，果期为9月至10月。黄连木的年生长过程中有三个快速生长阶段。然而，关于黄连木性别和生长季节相关的次生代谢产物的知识仍然匮乏。在本研究中，采用高效液相色谱法对雌雄树花序和叶片中的儿茶素水合物、芦丁、槲皮素和山奈酚含量进行定性和定量测定。还使用分光光度计检测了总酚和黄酮类化合物。结果表明，这些化合物的含量随季节波动，在新生叶片中达到最高水平。这些化合物的波动遵循不同的进化途径，在整个生长过程中，雌雄树中这些化合物的含量有增有减，因为它们具有各自的生物学功能。此外，提取物表现出清除DPPH自由基的生物活性，对3T3-L1前脂肪细胞无明显细胞毒性。综上所述，这些结果表明黄连木作为抗氧化药物具有巨大潜力，最佳收获时间是春季。
