Wang Huan, Hu Tian-Yu, Zhao Zhi-Tao, Zhang Xiu-Yun, Wang Ying, Duan Xiao-Qin, Liu Da-Wei, Jing Ling, Ma Qiang
Institute of Endemic Disease of Jilin University, Changchun 130021, China; The Second Hospital Norman Bethune of Jilin University, Changchun 130041, China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Talanta. 2016;149:285-289. doi: 10.1016/j.talanta.2015.12.001. Epub 2015 Dec 2.
In this paper, 3-aminobenzeneboronic acid functionalized Mn(2+)-doped ZnTe/ZnSe quantum dots (APBA-dQDs) were prepared. The APBA functional groups had strong binding ability with F(-), resulting in the quenchment of dQDs photoluminescence (PL). Under the optimal condition, the fluorescence intensity of APBA-dQDs was related linearly to the concentration of F(-) in the range of 0.25-1.5µmol/L with a detection limit of 0.1µmol/L. The selectivity of fluorescence quenching of APBA-dQDs for F(-) was enhanced. Moreover, the proposed methodology for the sensing of F(-) at EM 560nm in MC3T3-E1 osteoblastic cells was demonstrated and got a satisfactory results. The results indicate that the APBA-dQDs are promising candidates for intracellular in MC3T3-E1 osteoblastic cells. To the best of our knowledge, it was the first report of F(-) sensing by using the quenched fluorescence of APBA-dQDs in non-cancerous cells.
