Morita Naoki, Haga Sanae, Ohmiya Yoshihiro, Ozaki Michitaka
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Hokkaido 062-8517, Japan.
Laboratory of Molecular and Functional Bio-Imaging, Faculty of Health Sciences, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan.
Anal Biochem. 2016 Mar 15;497:24-6. doi: 10.1016/j.ab.2015.12.007. Epub 2015 Dec 22.
We propose a new concept of tumor progression monitoring using dual luciferases in living animals to reduce stress for small animals and the cost of luciferin. The secreted Cypridina luciferase (CLuc) was used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase was used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Thus, the new monitoring systems that use dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals.
我们提出了一种在活体动物中使用双荧光素酶监测肿瘤进展的新概念，以减轻小动物的应激反应并降低荧光素的成本。分泌型海萤荧光素酶（CLuc）用作体外指标，以持续监测肿瘤进展。另一方面，非分泌型萤火虫荧光素酶用作体内指标，在CLuc指示的合适时间点分析肿瘤的空间分布。因此，使用双荧光素酶的新型监测系统是可行的，可在对实验动物最小应激的情况下进行长期生物发光成像。
