用于监测多种启动子功能的分泌型报告基因。

Secreted Reporters for Monitoring Multiple Promoter Function.

作者信息

Lashgari Ghazal, Kantar Rami S, Tannous Bakhos A

机构信息

Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA.

Program in Neuroscience, Harvard Medical School, Boston, MA, USA.

出版信息

Methods Mol Biol. 2017;1651:33-47. doi: 10.1007/978-1-4939-7223-4_4.

DOI:10.1007/978-1-4939-7223-4_4

PMID:28801898

Abstract

Secreted reporter proteins are reliable modalities for monitoring of different biological processes, which can be measured longitudinally in conditioned medium of cultured cells or body fluids such as blood and urine, ex vivo. In this chapter, we will explore established secreted reporters and their applications and limitations for monitoring of promoter function. We will also describe both cell-based and blood-based assays for detecting three commonly used reporters: secreted alkaline phosphatase (SEAP ), Gaussia luciferase (Gluc), and Vargula luciferase (Vluc).

摘要

分泌型报告蛋白是监测不同生物学过程的可靠方式，可在培养细胞的条件培养基或血液和尿液等体液中进行纵向离体测量。在本章中，我们将探讨已有的分泌型报告基因及其在监测启动子功能方面的应用和局限性。我们还将描述用于检测三种常用报告基因的基于细胞和基于血液的检测方法：分泌型碱性磷酸酶（SEAP）、高斯荧光素酶（Gluc）和海萤荧光素酶（Vluc）。