酰基甘油组成和脂肪酰链长度对pH计消化模型及体外模拟消化模型中脂质消化的影响

Effect of Acylglycerol Composition and Fatty Acyl Chain Length on Lipid Digestion in pH-Stat Digestion Model and Simulated In Vitro Digestion Model.

作者信息

Qi Jin F, Jia Cai H, Shin Jung A, Woo Jeong M, Wang Xiang Y, Park Jong T, Hong Soon T, Lee K-T

机构信息

Dept. of Food Science and Technology, Chungnam National Univ, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, South Korea.

Sericultural Research Inst., Chinese Acad. of Agricultural Sciences, Jiangsu Univ. of Science and Technology, Zhenjiang, 212018, China.

出版信息

J Food Sci. 2016 Feb;81(2):C317-23. doi: 10.1111/1750-3841.13196. Epub 2015 Dec 31.

DOI:10.1111/1750-3841.13196

PMID:26720174

Abstract

In this study, a pH-stat digestion model and a simulated in vitro digestion model were employed to evaluate the digestion degree of lipids depending on different acylglycerols and acyl chain length (that is, diacylglycerol [DAG] compared with soybean oil representing long-chain triacylglycerol compared with medium-chain triacylglycerol [MCT]). In the pH-stat digestion model, differences were observed among the digestion degrees of 3 oils using digestion rate (k), digestion half-time (t1/2 ), and digestion extent (Φmax). The results showed the digestion rate order was MCT > soybean oil > DAG. Accordingly, the order of digestion half-times was MCT < soybean oil < DAG. In simulated in vitro digestion model, digestion rates (k') and digestion half-times (t'1/2 ) were also obtained and the results showed a digestion rate order of MCT (k' = 0.068 min(-1) ) > soybean oil (k' = 0.037 min(-1) ) > DAG (k' = 0.024 min(-1) ). Consequently, the order of digestion half-times was MCT (t'1/2 = 10.20 min) < soybean oil (t'1/2 = 18.74 min) < DAG (t'1/2 = 29.08 min). The parameters obtained using the 2 models showed MCT was digested faster than soybean oil, and that soybean oil was digested faster than DAG.

摘要

在本研究中，采用pH值恒定消化模型和体外模拟消化模型，根据不同的酰基甘油和酰基链长度（即二酰基甘油[DAG]与代表长链三酰基甘油的大豆油相比，与中链三酰基甘油[MCT]相比）来评估脂质的消化程度。在pH值恒定消化模型中，使用消化速率（k）、消化半衰期（t1/2）和消化程度（Φmax）观察到3种油的消化程度存在差异。结果表明，消化速率顺序为MCT>大豆油>DAG。因此，消化半衰期的顺序为MCT<大豆油<DAG。在体外模拟消化模型中，也获得了消化速率（k'）和消化半衰期（t'1/2），结果显示消化速率顺序为MCT（k' = 0.068 min-1）>大豆油（k' = 0.037 min-1）>DAG（k' = 0.024 min-1）。因此，消化半衰期的顺序为MCT（t'1/2 = 10.20 min）<大豆油（t'1/2 = 18.74 min）<DAG（t'1/2 = 29.08 min）。使用这两种模型获得的参数表明，MCT的消化速度比大豆油快，大豆油的消化速度比DAG快。