Deng Liang, Li Dejian, Gu Wenguang, Liu Aiguo, Cheng Xiangyang
Department of Orthopedic Surgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Oncol Lett. 2015 Nov;10(5):3323-3331. doi: 10.3892/ol.2015.3712. Epub 2015 Sep 16.
Various human cancers have been revealed to contain cancer stem-like cells (CSCs) and the spherical colonies that possess stem-like properties and cancer-initiating abilities. Malignant fibrous histiocytoma (MFH) is a common soft-tissue sarcoma, and is considered to be a myxoma due to the observed high-grade lesions. In the present study, the spherical colonies were isolated from a human MFH cell line NMFH-1 using the sphere culture system. These colonies demonstrated stem-like properties, with the ability of self-renewal and strong drug-resistance to doxorubicin and cisplatin. In addition, verapamil, an adenosine triphosphate binding cassette protein transporter protein (ABCG2) inhibitor, enhanced the efficacy of the aforementioned chemotherapy agents. These colonies also demonstrated an increased expression of embryonic stem genes, including Oct3/4, signal transducer and activator of transcription 3, sex determining region Y-box 10 and ABCG2, and stem cell-associated surface markers, such as cluster of differentiation (CD)44 and CD133. These results indicated that NMFH-1 lesions contain cancer stem-like cell populations that demonstrate strong drug resistance, and verapamil enhanced the efficacy of the chemotherapy agents.
已发现多种人类癌症含有癌干细胞样细胞（CSCs）以及具有干细胞样特性和癌症起始能力的球形集落。恶性纤维组织细胞瘤（MFH）是一种常见的软组织肉瘤，由于观察到高级别病变，被认为是一种黏液瘤。在本研究中，使用球体培养系统从人MFH细胞系NMFH-1中分离出球形集落。这些集落表现出干细胞样特性，具有自我更新能力以及对阿霉素和顺铂的强耐药性。此外，维拉帕米，一种三磷酸腺苷结合盒转运蛋白（ABCG2）抑制剂，增强了上述化疗药物的疗效。这些集落还表现出胚胎干细胞基因的表达增加，包括Oct3/4、信号转导和转录激活因子3、性别决定区Y盒10和ABCG2，以及干细胞相关表面标志物，如分化簇（CD）44和CD133。这些结果表明，NMFH-1病变含有表现出强耐药性的癌干细胞样细胞群体，并且维拉帕米增强了化疗药物的疗效。
