Institute of Orthopaedics and Traumatology of PLA of China, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, PR China.
Biochem Biophys Res Commun. 2010 Jan 1;391(1):1062-6. doi: 10.1016/j.bbrc.2009.12.020. Epub 2009 Dec 10.
Dye exclusion is a valuable technique to isolate cancer stem cells (CSCs) based on an ability of stem cell to efflux fluorescent DNA-binding dye, especially for tumors without unique surface markers. It has been proven that side population (SP) cells that exclude Hoechst 33342 dye are enriched with stem-like cells in several cancer cell lines. In this study, we isolated and characterized SP cells from human Ewing's sarcoma cell line SK-ES-1 in vitro. SP cells were detected in SK-ES-1 and comprised 1.2% of total cell population. Only SP cells had the capacity to regenerate both SP and non-SP cells. The proliferation rates were similar between SP and non-SP cells. However, the clonogenicity and invasiveness of SP cells were significantly higher than that of non-SP cells. Further characterization of this SP phenotype presented other properties. SP cells exhibited increased multi-drug resistance and the ATP binding cassette protein (ABC) transporters were up-regulated in SP population. These findings suggest that SP cells derived from Ewing's sarcoma play the critical role in tumor metastasis and recurrence and might be an ideal target for clinical therapy.
染料排除法是一种基于干细胞能够排出荧光 DNA 结合染料的能力来分离癌症干细胞 (CSC) 的有价值的技术，特别是对于没有独特表面标记的肿瘤。已经证明，几种癌细胞系中的侧群 (SP) 细胞排斥 Hoechst 33342 染料，富含干细胞样细胞。在这项研究中，我们在体外从人尤文肉瘤细胞系 SK-ES-1 中分离和鉴定了 SP 细胞。SP 细胞在 SK-ES-1 中被检测到，占总细胞群体的 1.2%。只有 SP 细胞具有再生 SP 和非 SP 细胞的能力。SP 细胞和非 SP 细胞的增殖率相似。然而，SP 细胞的克隆形成能力和侵袭性明显高于非 SP 细胞。对这种 SP 表型的进一步特征分析呈现出其他特性。SP 细胞表现出增加的多药耐药性，并且 SP 群体中 ABC 转运蛋白上调。这些发现表明，源自尤文肉瘤的 SP 细胞在肿瘤转移和复发中起关键作用，可能是临床治疗的理想靶点。
