Departamento de Química, Universidade Estadual de Maringá, Avenida Colombo 5790, CEP: 87020-900 Maringá, Paraná, Brazil.
Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Avenida Colombo 5790, CEP: 87020-900 Maringá, Paraná, Brazil.
J Colloid Interface Sci. 2016 Mar 15;466:150-61. doi: 10.1016/j.jcis.2015.12.016. Epub 2015 Dec 9.
A facile and reproducible route that can lead to two-dimensional arrays of nanopores in thin polymer films is demonstrated. The formation of the pores in the polymer films involves breath figure phenomenon and occurs during the film deposition by spin coating. The formation of nanoporous thin films takes only few seconds, and the method does not require complex equipment or expensive chemicals. This method also constitutes a straightforward approach to control the size of the pores formed in thin films. Besides allowing control over the average pore size of the porous films, the use of dynamic deposition with the breath figure phenomenon causes the reduction in the pore size to nanometer scale. The nanoporous arrays obtained by the breath figure are applied as substrates for cell growth, and the effect of their nanopore size on cell growth was evaluated. Notably, it is found that cell viability is related to pore size, where 2D nanoporous structure is more beneficial for cell culture than 2D microporous structures. The change in the average pore size of the polymer films from 1.22 μm to 346 nm results in a threefold increase in cell viability.
一种简单且可重现的方法可以在聚合物薄膜中形成二维纳米孔阵列。聚合物薄膜中孔的形成涉及呼吸图案现象，并且在通过旋涂进行薄膜沉积期间发生。纳米多孔薄膜的形成仅需几秒钟，并且该方法不需要复杂的设备或昂贵的化学物质。这种方法还构成了控制薄膜中形成的孔的尺寸的直接方法。除了允许控制多孔膜的平均孔径之外，使用呼吸图案的动态沉积导致孔径减小到纳米级。通过呼吸图案获得的纳米多孔阵列被用作细胞生长的基底，并评估了其纳米孔尺寸对细胞生长的影响。值得注意的是，发现细胞活力与孔径有关，其中二维纳米多孔结构比二维微孔结构更有利于细胞培养。聚合物膜的平均孔径从 1.22μm 变化到 346nm 导致细胞活力增加了三倍。
