Rad Hamed Mirzaei, Eslami Mohsen, Ghanie Abolfazl
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Anim Reprod Sci. 2016 Feb;165:38-45. doi: 10.1016/j.anireprosci.2015.12.003. Epub 2015 Dec 12.
The practice of artificial insemination is widely utilized in poultry; and this requires a broad use of semen storage techniques to prevent the reduction of fertilizing ability of stored semen. The antioxidant activity of palmitoleic acid with in vitro experiments has been shown. The present study was designed to evaluate the effect of palmitoleic acid on the quality of rooster semen stored at 4C. Semen was collected from ten roosters twice a week. Ejaculates with greater than 80% forward spermatozoa motility were pooled and after dilution semen was enriched with 0 (control), 0.125 (P 0.125), 0.25 (P 0.25), 0.5 (P 0.5) and 1 (P 1) millimolar palmitoleate. Forward spermatozoa progressive motility and viability, as well as amounts of malondialdehyde (MDA) and total antioxidant activity (AOA) were evaluated in seminal plasma and spermatozoa at 0, 24 and 48h of storage. Motility was 78.5±2.21, 77.5±1.04, and 69.5±2.32% at 24h and 58.66±1.35, 49.33±1.36 and 43.00±2.08% at 48h in P 0.125, P 0.25 and control, respectively (P<0.02). There were no significant differences in amount of MDA in the seminal plasma among groups, while the amounts of MDA in spermatozoa were less in the P 0.125, P 0.25 and P 0.5 groups compared to the control group at 24 and 48h of storage (P<0.002). Total amounts of AOA in seminal plasma were greater in palmitoleate treatment groups than the control at 24 and 48h (P<0.01). Moreover, palmitoleate treatment groups had greater values of total AOA in spermatozoa compared to the control group at 24 and 48h of storage (P<0.05). In conclusion, enrichment of rooster semen with small doses of palmitoleate has beneficial effects on the semen quality during cold storage.
人工授精技术在禽类养殖中广泛应用；这需要广泛运用精液保存技术以防止储存精液的受精能力下降。体外实验已表明棕榈油酸的抗氧化活性。本研究旨在评估棕榈油酸对4℃储存的公鸡精液质量的影响。每周从十只公鸡采集两次精液。将精子前向运动率大于80%的射精精液汇集，稀释后精液分别添加0（对照组）、0.125（P 0.125组）、0.25（P 0.25组）、0.5（P 0.5组）和1（P 1组）毫摩尔的棕榈油酸酯。在储存0、24和48小时时，评估精浆和精子中的精子前向运动性、活力以及丙二醛（MDA）含量和总抗氧化活性（AOA）。在24小时时，P 0.125组、P 0.25组和对照组的精子运动率分别为78.5±2.21%、77.5±1.04%和69.5±2.32%；在48小时时，分别为分别为58.66±1.35%、49.33±1.36%和43.00±2.08%（P<0.02）。各组精浆中MDA含量无显著差异，而在储存24和48小时时，P 0.125组、P 0.25组和P 0.5组精子中的MDA含量低于对照组（P<0.002）。在24和48小时时，棕榈油酸酯处理组精浆中的总AOA含量高于对照组（P<0.01）。此外，在储存24和48小时时，棕榈油酸酯处理组精子中的总AOA值高于对照组（P<0.05）。总之，小剂量棕榈油酸酯富集公鸡精液对冷藏期间的精液质量有有益影响。
