Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, 17166 Tehran, Iran.
Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, 17166 Tehran, Iran.
Poult Sci. 2020 Mar;99(3):1678-1686. doi: 10.1016/j.psj.2019.12.006. Epub 2020 Jan 24.
The protective role of astaxanthin nanoparticles (Ast NPs, 25 mg/kg p.o) against cadmium (Cd, 1 mg/100 g b.w. SC), a known inductor of lipid peroxidation and changes in the antioxidant defense system in the Ross 308 breeder roosters sperm, was examined. Sperm motility (computer-assisted sperm motility analysis), membrane integrity (hypoosmotic swelling test), viability, total abnormality, and enzymatic parameters were assessed after thawing. The testis/body weight (mg/kg) ratio and HE staining results of testis were also performed. The obtained results showed that Cd induced detrimental effects on testis and sperm, while Cd treated by Ast NPs (Cd Ast) diminished this change compared to the Cd group. Cd-treated group resulted in significantly (P < 0.05) lowest total (37.29 ± 2.46) and progressive (5.84 ± 0.47) motility and decreased antioxidant enzyme activity (CAT, TAC, and GPx), as well as producing a significant (P < 0.05) decrease in testis weight (mg) compared to the control group. Treatment with Ast NPs (Ast NPs + Cd) had reversed Cd-induced changes in the antioxidant defense system and significantly prevented Cd-induced testis damage. In conclusion, the results of our work suggest that Ast NPs at 25 mg/kg act as a potent antioxidant in protecting rooster testes against oxidative stress induced by Cd.
虾青素纳米粒子（Ast NPs，25mg/kg，po）对镉（Cd，1mg/100g bw，sc）的保护作用，镉是一种已知的脂质过氧化诱导剂和抗氧化防御系统变化的诱导剂，在罗斯 308 种公鸡精子中，进行了检查。解冻后评估精子活力（计算机辅助精子活力分析）、膜完整性（低渗肿胀试验）、活力、总异常和酶学参数。还进行了睾丸/体重（mg/kg）比值和睾丸 HE 染色结果的检测。结果表明，Cd 对睾丸和精子有不良影响，而用 Ast NPs 处理的 Cd（Cd Ast）与 Cd 组相比，减轻了这种变化。与对照组相比，Cd 处理组的总（37.29±2.46）和渐进（5.84±0.47）活力以及抗氧化酶活性（CAT、TAC 和 GPx）显著降低（P<0.05），睾丸重量（mg）也显著降低（P<0.05）。用 Ast NPs（Ast NPs+Cd）处理可逆转抗氧化防御系统中由 Cd 诱导的变化，并显著防止 Cd 诱导的睾丸损伤。总之，我们的工作结果表明，25mg/kg 的 Ast NPs 作为一种有效的抗氧化剂，可防止 Cd 诱导的公鸡睾丸氧化应激。
