Zhu Binglin, Zha Jingsi, Long Yan, Hu Xiaotong, Chen Guojun, Wang Xuefeng
Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China.
Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China.
J Neurol Sci. 2016 Jan 15;360:30-6. doi: 10.1016/j.jns.2015.11.041. Epub 2015 Nov 22.
Copine VI (CPNE6) is a member of copines family, a calcium-dependent phospholipids-binding protein group found in many diverse eukaryotic organisms. Although earlier studies have shown that CPNE6 is almost exclusively expressed in brain, the exact biological functions remain unclear. The purpose of this study is to explore the relationship between epilepsy and CPNE6 expression. In present study, we investigated the expression pattern and distribution of CPNE6 in patients with refractory epilepsy and in a chronic pilocarpine-induced epileptic rat model by quantitative real-time PCR, Western blot and immunofluorescence. The results showed that the expression of CPNE6 increased remarkably in epileptic patients and in experimental epileptic rats. Double immunofluorescence labeling studies have revealed that CPNE6 protein is mainly expressed in neurons, demonstrated by co-localization with the dendritic marker, MAP2. Our results are the first to indicate that the abnormal expression of the CPNE6 in epileptic brain tissue may play an important role in epilepsy, especially refractory epilepsy.
柯平蛋白VI（CPNE6）是柯平蛋白家族的一员，柯平蛋白家族是一类在多种真核生物中发现的钙依赖性磷脂结合蛋白。尽管早期研究表明CPNE6几乎只在大脑中表达，但其确切的生物学功能仍不清楚。本研究的目的是探讨癫痫与CPNE6表达之间的关系。在本研究中，我们通过定量实时PCR、蛋白质免疫印迹和免疫荧光技术，研究了CPNE6在难治性癫痫患者和慢性匹鲁卡品诱导的癫痫大鼠模型中的表达模式和分布。结果显示，CPNE6在癫痫患者和实验性癫痫大鼠中的表达显著增加。双重免疫荧光标记研究表明，CPNE6蛋白主要在神经元中表达，这通过与树突标记物微管相关蛋白2（MAP2）的共定位得以证明。我们的结果首次表明，癫痫脑组织中CPNE6的异常表达可能在癫痫尤其是难治性癫痫中起重要作用。
