Seedevi Palaniappan, Moovendhan Meivelu, Vairamani Shanmugam, Shanmugam Annian
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
Int J Biol Macromol. 2016 Apr;85:117-25. doi: 10.1016/j.ijbiomac.2015.12.066. Epub 2015 Dec 24.
Sulfated polysaccharide was extracted from the internal shell (gladius) of Sepioteuthis lessoniana. The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively. The elemental composition was analyzed using CHNS/O analyzer. The molecular weight of sulfated polysaccharide determined through PAGE was found to be as 66 kDa. Monosaccharides analysis revealed that sulfated polysaccharide was composed of rhamnose, galactose, xylose and glucose. The structural features of sulfated polysaccharide were analyzed by FT-IR and NMR spectroscopy. Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method. The polysaccharide has showed good antibacterial activity and MIC and MBC have also been evaluated. The anticancer activity was tested against HeLa cell line by MTT assay. The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml. In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
从莱氏拟乌贼的内壳（海螵蛸）中提取了硫酸化多糖。该硫酸化多糖分别含有61.3%的碳水化合物、0.8%的蛋白质、28.2%的灰分和1.33%的水分。使用CHNS/O分析仪分析了其元素组成。通过PAGE测定的硫酸化多糖的分子量为66 kDa。单糖分析表明，硫酸化多糖由鼠李糖、半乳糖、木糖和葡萄糖组成。通过FT-IR和NMR光谱分析了硫酸化多糖的结构特征。此外，采用琼脂孔扩散法评估了该硫酸化多糖对选定的人类临床病原体的抗菌活性，这些病原体包括金黄色葡萄球菌、肺炎克雷伯菌、伤寒沙门氏菌、霍乱弧菌、产酸克雷伯菌、大肠杆菌、副伤寒沙门氏菌、奇异变形杆菌、副溶血性弧菌和化脓性链球菌。该多糖显示出良好的抗菌活性，同时也评估了其最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。通过MTT法对HeLa细胞系进行了抗癌活性测试。观察到细胞毒性浓度（CC50）为700 μg/ml，在200 μg/ml时记录到最大抗癌活性为62.89%；而在25 μg/ml时观察到最低活性为9.87%。总之，硫酸化多糖是一种替代性的、无毒且廉价的物质来源，具有良好的抗菌和抗癌活性。
