Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
Carbohydr Polym. 2017 Jul 1;167:129-135. doi: 10.1016/j.carbpol.2017.03.028. Epub 2017 Mar 11.
The sulfated mucopolysaccharide (GAG) was isolated from S. pharonis and the carbohydrate and protein content was found to be 62.4% and 3.9%. The disaccharide profile of sulfated GAG composed glucuronic acid, N-acetyl glucosamine and sulfate content by contributing 50.11%, 38.00% and 27.69% respectively. The carbon, hydrogen and nitrogen content of the sulfated GAG showed 14.80%, 1.68% and 2.99% respectively. The molecular weight of sulfated GAG was calculated as 27kDa and the structural characterization was done by Fourier Transform Infrared (FT-IR) and NMR Spectroscopy. The Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) of sulfated GAG were determined as 91 IU and 39.55 IU at 25μg/ml respectively. Further the sulfated GAG reported the cytotoxic effect (CC) of 1100μg/ml concentration on Vero cell line. The sulfated GAG reported the anticancer activity against HeLa cell line with an inhibition rate of 18.65%-66.13% at 50-250μg/ml concentration. The sulfated GAG can be considered as a potent anticoagulant and anticancer drug in future.
从 S. pharonis 中分离出硫酸化粘多糖（GAG），其糖和蛋白质含量分别为 62.4%和 3.9%。硫酸化 GAG 的二糖组成分别为葡萄糖醛酸、N-乙酰葡萄糖胺和硫酸，贡献比例为 50.11%、38.00%和 27.69%。硫酸化 GAG 的碳、氢和氮含量分别为 14.80%、1.68%和 2.99%。硫酸化 GAG 的分子量计算为 27kDa，并通过傅里叶变换红外（FT-IR）和 NMR 光谱进行结构表征。硫酸化 GAG 的活化部分凝血活酶时间（APTT）和凝血酶原时间（PT）分别在 25μg/ml 时为 91IU 和 39.55IU。此外，硫酸化 GAG 报告称在 1100μg/ml 浓度下对 Vero 细胞系具有细胞毒性作用（CC）。硫酸化 GAG 对 HeLa 细胞系具有抗癌活性，在 50-250μg/ml 浓度下抑制率为 18.65%-66.13%。硫酸化 GAG 将来可以被认为是一种有效的抗凝和抗癌药物。
