Pashintseva L P, Gudima S O, Kozyreva E A, Voronov A V, Mierinia A A
Vopr Med Khim. 1989 May-Jun;35(3):68-71.
Activity of placental alkaline phosphatase (PAP) was studied in blood serum of 53 healthy persons and of 72 oncologic patients, using solid-phase immunoenzymatic analysis with polyclonal antibodies towards PAP. The enzyme was detected both in blood serum of healthy persons and of oncologic patients. The blood serum under study was preheated at 65 degrees in order to inactivate the intestinal phosphatase--the only isoenzyme cross-reacting with antibodies to thermostable PAP. This controlled heating treatment decreased distinctly the possibility of pseudo-positive reactions. The limiting values of the PAP activity were about 0.15 un/L in blood sera of healthy persons. Higher values were considered as an evidence of pathological state. After screening analysis of blood sera from patients with various forms of malignant tumors the PAP activity above 0.15 un/L was observed in 20% of the patients; the enzymatic activity exceeded these values in 54% of patients with ovary carcinoma. The data obtained suggest that the procedure developed as an adequate means for estimation of thermostable PAP isoenzymes as well as that the rate of PAP activity might serve as marker of malignancy in ovary and testis carcinomas independently on level of total activity of alkaline phosphatase.
采用针对胎盘碱性磷酸酶（PAP）的多克隆抗体固相免疫酶分析法，对53名健康人和72名肿瘤患者的血清中PAP活性进行了研究。在健康人和肿瘤患者的血清中均检测到了该酶。为使肠道磷酸酶失活（肠道磷酸酶是唯一与抗热稳定PAP抗体发生交叉反应的同工酶），将所研究的血清在65摄氏度下预热。这种可控的加热处理显著降低了假阳性反应的可能性。健康人血清中PAP活性的极限值约为0.15单位/升。较高的值被视为病理状态的证据。在对各种形式恶性肿瘤患者的血清进行筛查分析后，20%的患者观察到PAP活性高于0.15单位/升；在54%的卵巢癌患者中，酶活性超过了这些值。所获得的数据表明，所开发的程序是评估热稳定PAP同工酶的适当手段，并且PAP活性速率可能作为卵巢癌和睾丸癌恶性肿瘤的标志物，与碱性磷酸酶总活性水平无关。
