The Department of Ophthalmology, Faculty of Medicine, Sohag University, Sohag, Egypt.
The Department of Biochemistry, Faculty of Medicine, Sohag University, Sohag, Egypt.
Acta Ophthalmol. 2016 Aug;94(5):e361-6. doi: 10.1111/aos.12919. Epub 2016 Jan 4.
To elucidate the role of heat shock protein-70 (HSP70) and hypoxia inducible factor-1α (HIF-1α) in diabetic retinopathy (DR) patients.
A comparative study was done on the serum level of both HSP70 and HIF-1α in 50 patients with type 2 diabetes mellitus (T2DM) without DR, 50 patients with T2DM and DR and 70 healthy control subjects.
HSP70 and HIF-1α were significantly increased in T2DM patients compared to controls and increased in patients with T2DM & DR compared to T2DM patients without DR (p < 0.0001). HSP70 did not differ among the patients with different stages of DR, while HIF-1α increased significantly in grades 3 and 4 DR patients compared to grades 1 and 2 DR patients. A strong correlation was found between HIF-1α and the development of DR (r = 0.835, p = 0.00) but not with HSP70. HIF-1α can be used as a predictor for development of DR but not HSP70.
Our study was the first that investigated both HSP70 and HIF-1α in humans and was the first that measured their levels in serum of patients with DR. The study suggested that HSP70 might have a protective function in T2DM patients rather than a therapeutic function. HIF-1α had an upper hand in the development and progression of DR. Induction of HSP70 and blockage of HIF-1α could lead to the development of novel prophylactic and therapeutic strategies for DR and potentially other diabetic complications.
阐明热休克蛋白 70（HSP70）和缺氧诱导因子 1α（HIF-1α）在糖尿病视网膜病变（DR）患者中的作用。
对 50 例 2 型糖尿病（T2DM）无 DR 患者、50 例 T2DM 合并 DR 患者和 70 例健康对照者的血清 HSP70 和 HIF-1α水平进行了比较研究。
与对照组相比，T2DM 患者的 HSP70 和 HIF-1α明显升高，T2DM 合并 DR 患者的 HSP70 和 HIF-1α明显高于 T2DM 无 DR 患者（均 P＜0.0001）。不同 DR 分期患者的 HSP70 无差异，而 3 级和 4 级 DR 患者的 HIF-1α明显高于 1 级和 2 级 DR 患者。HIF-1α与 DR 的发生有很强的相关性（r＝0.835，P＝0.00），而与 HSP70 无相关性。HIF-1α可作为预测 DR 发生的指标，但 HSP70 不行。
本研究首次在人类中同时检测了 HSP70 和 HIF-1α，并首次测量了它们在 DR 患者血清中的水平。研究表明，HSP70 在 T2DM 患者中可能具有保护作用，而不是治疗作用。HIF-1α在 DR 的发生和进展中占主导地位。诱导 HSP70 和阻断 HIF-1α可能导致针对 DR 及潜在其他糖尿病并发症的新的预防和治疗策略的发展。
