Chernesky M A
McMaster University Regional Virology and Chlamydiology Laboratories, St Joseph's Hospital, Hamilton, Ontario, Canada.
Yale J Biol Med. 1989 Mar-Apr;62(2):89-92.
The earliest laboratory diagnoses of viral infections were made by microscopy just after the turn of the twentieth century. Animal and egg inoculation were the methods of choice until tissue culture and serology accelerated the field of diagnostic virology during the fifties and sixties. More rapid methods, including electron microscopy, immunoassays, and nucleic acid probes, are now available and influencing laboratory decisions and patient care. This review discusses changes in science and society which have influenced diagnostic virology and how the discipline has responded to these influences.
20世纪之交后不久，最早通过显微镜对病毒感染进行实验室诊断。在50年代和60年代组织培养和血清学加速诊断病毒学领域的发展之前，动物接种和鸡胚接种是首选方法。现在已有包括电子显微镜、免疫测定和核酸探针在内的更快速的方法，这些方法正在影响实验室决策和患者护理。本文综述了影响诊断病毒学的科学和社会变化，以及该学科对这些影响的应对方式。
