Park Bong Joo, Choi Kyong-Hoon, Nam Ki Chang, Min Jeeeun, Lee Kyu-Dong, Uhm Han Sup, Choi Eun Ha, Kim Ho-Joong, Jung Jin-Seung
J Nanosci Nanotechnol. 2015 Oct;15(10):7900-6. doi: 10.1166/jnn.2015.11236.
This work reports the synthesis and the characterization of water-soluble and biocompatible photosensitizer (PS)-conjugated magnetic nanoparticles composed of a cobalt ferrite (CoFe2O4) magnetic core coated with a biocompatible hematoporphyrin (HP) shell. The photo-functional cobalt ferrite magnetic nanoparticles (CoFe2O4@HP) were uniform in size, stable against PS leaching, and highly efficient in the photo-generation of cytotoxic singlet oxygen under visible light. With the CoFe2O4@HP, we acquired in vitro MR images of cancer cells (PC-3) and confirmed good biocompatibility of the CoFe2O4@HP in both normal and cancer cells. In addition, we confirmed the potential of the CoFe2O4@HP as an agent for photodynamic therapy (PDT) applications. The photodynamic anticancer activities in 25, 50, and 100 μg/mL of CoFe2O4@HP were measured and found to exceed 99% (99.0, 99.4, and 99.5%) (p < 0.002). The photodynamic anticancer activity was 81.8% (p < 0.003). From these results, we suggest that our CoFe2O4@HP can be used safely as a type of photodynamic cancer therapy with potential as a therapeutic agent having good biocompatibility. Moreover, these photo-functional magnetic nanoparticles are highly promising for applications in versatile imaging diagnosis and as a therapy tool in biomedical engineering.
本研究报告了一种水溶性且具有生物相容性的光敏剂（PS）共轭磁性纳米颗粒的合成与表征，该纳米颗粒由包覆有生物相容性血卟啉（HP）壳层的钴铁氧体（CoFe2O4）磁芯组成。光功能化的钴铁氧体磁性纳米颗粒（CoFe2O4@HP）尺寸均匀，PS浸出稳定，在可见光下产生细胞毒性单线态氧的效率很高。利用CoFe2O4@HP，我们获得了癌细胞（PC-3）的体外磁共振图像，并证实了CoFe2O4@HP在正常细胞和癌细胞中均具有良好的生物相容性。此外，我们证实了CoFe2O4@HP作为光动力疗法（PDT）应用试剂的潜力。测量了25、50和100μg/mL的CoFe2O4@HP的光动力抗癌活性，发现其超过99%（99.0、99.4和99.5%）（p<0.002）。光动力抗癌活性为81.8%（p<0.003）。基于这些结果，我们认为我们的CoFe2O4@HP可以安全地用作一种光动力癌症治疗方法，具有作为具有良好生物相容性的治疗剂的潜力。此外，这些光功能化磁性纳米颗粒在多功能成像诊断以及作为生物医学工程中的治疗工具方面具有高度的应用前景。
