Mao Xiangbing, Gu Changsong, Hu Haiyan, Tang Jun, Chen Daiwen, Yu Bing, He Jun, Yu Jie, Luo Junqiu, Tian Gang
Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China.
Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.
PLoS One. 2016 Jan 4;11(1):e0146312. doi: 10.1371/journal.pone.0146312. eCollection 2016.
Lactobacillus rhamnosus GG (LGG) has been regarded as a safe probiotic strain. The aim of this study was to investigate whether dietary LGG supplementation could alleviate diarrhea via improving jejunal mucosal barrier function in the weaned piglets challenged by RV, and further analyze the potential roles for apoptosis of jejunal mucosal cells and intestinal microbiota. A total of 24 crossbred barrows weaned at 21 d of age were assigned randomly to 1 of 2 diets: the basal diet and LGG supplementing diet. On day 11, all pigs were orally infused RV or the sterile essential medium. RV infusion increased the diarrhea rate, increased the RV-Ab, NSP4 and IL-2 concentrations and the Bax mRNA levels of jejunal mucosa (P<0.05), decreased the villus height, villus height: crypt depth, the sIgA, IL-4 and mucin 1 concentrations and the ZO-1, occludin and Bcl-2 mRNA levels of jejunal mucosa (P<0.05), and affected the microbiota of ileum and cecum (P<0.05) in the weaned pigs. Dietary LGG supplementation increased the villus height and villus height: crypt depth, the sIgA, IL-4, mucin 1 and mucin 2 concentrations, and the ZO-1, occludin and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05) reduced the Bax mRNA levels of the jejunal mucosa (P<0.05) in weaned pigs. Furthermore, dietary LGG supplementation alleviated the increase of diarrhea rate in the weaned pigs challenged by RV (P<0.05), and relieve the effect of RV infection on the villus height, crypt depth and the villus height: crypt depth of the jejunal mucosa (P<0.05), the NSP4, sIgA, IL-2, IL-4, mucin 1 and mucin 2 concentrations of jejunal mucosa (P<0.05), the ZO-1, occludin, Bax and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05), and the microbiota of ileum and cecum (P<0.05) in the weaned pigs challenged by RV. These results suggest that supplementing LGG in diets alleviated the diarrhea of weaned piglets challenged by RV via inhibiting the virus multiplication and improving the jejunal mucosal barrier function, which was possibly due to the decreasing apoptosis of jejunal mucosal cells and the improvement of intestinal microbiota.
鼠李糖乳杆菌GG（LGG）被认为是一种安全的益生菌菌株。本研究的目的是探讨日粮中添加LGG是否能通过改善空肠黏膜屏障功能来缓解轮状病毒（RV）感染的断奶仔猪腹泻，并进一步分析空肠黏膜细胞凋亡和肠道微生物群的潜在作用。选取24头21日龄断奶的杂交公猪，随机分为2组，分别饲喂基础日粮和添加LGG的日粮。在第11天，所有仔猪经口灌喂RV或无菌基础培养基。灌喂RV增加了断奶仔猪的腹泻率、RV抗体、NSP4和IL-2浓度以及空肠黏膜的Bax mRNA水平（P<0.05），降低了空肠黏膜的绒毛高度、绒毛高度与隐窝深度比值、sIgA、IL-4和黏蛋白1浓度以及ZO-1、闭合蛋白和Bcl-2 mRNA水平（P<0.05），并影响了回肠和盲肠的微生物群（P<0.05）。日粮中添加LGG增加了断奶仔猪空肠黏膜的绒毛高度、绒毛高度与隐窝深度比值、sIgA、IL-4、黏蛋白1和黏蛋白2浓度以及ZO-1、闭合蛋白和Bcl-2 mRNA水平（P<0.05），降低了空肠黏膜的Bax mRNA水平（P<0.05）。此外，日粮中添加LGG缓解了RV感染的断奶仔猪腹泻率的升高（P<0.05），减轻了RV感染对空肠黏膜绒毛高度、隐窝深度以及绒毛高度与隐窝深度比值（P<0.05）、空肠黏膜NSP4、sIgA、IL-2、IL-4、黏蛋白1和黏蛋白2浓度（P<0.05）、空肠黏膜ZO-1、闭合蛋白、Bax和Bcl-2 mRNA水平（P<0.05）以及回肠和盲肠微生物群（P<0.05）的影响。这些结果表明，日粮中添加LGG通过抑制病毒增殖和改善空肠黏膜屏障功能缓解了RV感染的断奶仔猪腹泻，这可能是由于空肠黏膜细胞凋亡减少和肠道微生物群改善所致。
