Zhang Husen, Wang Haifeng, Shepherd Megan, Wen Ke, Li Guohua, Yang Xingdong, Kocher Jacob, Giri-Rachman Ernawati, Dickerman Allan, Settlage Robert, Yuan Lijuan
Department of Civil and Environmental Engineering, Virginia Polytechnic Institute and State University, Blacksburg 24061, VA, USA.
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061, VA, USA ; College of Animal Science & Technology, Zhejiang A & F University, Zhejiang Province, China.
Gut Pathog. 2014 Sep 9;6:39. doi: 10.1186/s13099-014-0039-8. eCollection 2014.
We generated a neonatal pig model with human infant gut microbiota (HGM) to study the effect of a probiotic on the composition of the transplanted microbiota following rotavirus vaccination and challenge. All the HGM-transplanted pigs received two doses of an oral attenuated rotavirus vaccine. The gut microbiota of vaccinated pigs were investigated for effects of Lactobacillus rhamnosus GG (LGG) supplement and homotypic virulent human rotavirus (HRV) challenge. High-throughput sequencing of V4 region of 16S rRNA genes demonstrated that HGM-transplanted pigs carried microbiota similar to that of the C-section delivered baby. Firmicutes and Proteobacteria represented over 98% of total bacteria in the human donor and the recipient pigs. HRV challenge caused a phylum-level shift from Firmicutes to Proteobacteria. LGG supplement prevented the changes in microbial communities caused by HRV challenge. In particular, members of Enterococcus in LGG-supplemented pigs were kept at the baseline level, while they were enriched in HRV challenged pigs. Taken together, our results suggested that HGM pigs are valuable for testing the microbiota's response to probiotic interventions for treating infantile HRV infection.
我们构建了一种具有人类婴儿肠道微生物群（HGM）的新生猪模型，以研究益生菌对轮状病毒疫苗接种和攻击后移植微生物群组成的影响。所有移植了HGM的猪都接受了两剂口服减毒轮状病毒疫苗。研究了接种疫苗的猪的肠道微生物群对鼠李糖乳杆菌GG（LGG）补充剂和同型强毒人类轮状病毒（HRV）攻击的影响。对16S rRNA基因V4区域的高通量测序表明，移植了HGM的猪携带的微生物群与剖宫产出生的婴儿相似。厚壁菌门和变形菌门在人类供体和受体猪的总细菌中占比超过98%。HRV攻击导致了从厚壁菌门到变形菌门的门类水平转变。补充LGG可防止HRV攻击引起的微生物群落变化。特别是，补充LGG的猪中的肠球菌成员保持在基线水平，而在HRV攻击的猪中则富集。综上所述，我们的结果表明，HGM猪对于测试微生物群对治疗婴儿HRV感染的益生菌干预的反应具有重要价值。
