Xu Yameng, Ji Quanbo, Zhang Qiang, Wang Yan
Department of Traditional Chinese Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China.
Department of Orthopaedics, 301 Hospital, Beijing 100853, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Jan;32(1):29-33.
To explore the effect of cryptotanshinone (CTS) on vascular endothelial growth factor (VEGF) expression in U2OS osteosarcoma cells, with a focus on angiogenesis.
U2OS osteosarcoma cells cultured in vitro were divided into (20, 40, 80, 160) μmol/L CTS treated groups and control group. Real-time quantitative PCR was performed to detect the expression of VEGF mRNA in U2OS osteosarcoma cells. The VEGF protein level was determined using Western blotting and immunofluorescence cytochemistry. Cell proliferation ability was detected by CCK-8 assay. The tube formation assay in vitro was used to observe the angiogenesis ability.
CTS inhibited the levels of VEGF mRNA and protein in a dose-dependent manner in U2OS osteosarcoma cells obviously. CCK-8 assay indicated that CTS inhibited the proliferation of U2OS osteosarcoma cells. The tube formation assay in vitro revealed that CTS inhibited the angiogenesis ability.
CTS could down-regulate the expression of VEGF and inhibit angiogenesis in U2OS osteosarcoma cells.
探讨隐丹参酮（CTS）对U2OS骨肉瘤细胞中血管内皮生长因子（VEGF）表达的影响，重点关注血管生成。
将体外培养的U2OS骨肉瘤细胞分为（20、40、80、160）μmol/L CTS处理组和对照组。采用实时定量PCR检测U2OS骨肉瘤细胞中VEGF mRNA的表达。使用蛋白质印迹法和免疫荧光细胞化学法测定VEGF蛋白水平。通过CCK-8法检测细胞增殖能力。采用体外管腔形成试验观察血管生成能力。
CTS明显以剂量依赖性方式抑制U2OS骨肉瘤细胞中VEGF mRNA和蛋白水平。CCK-8法表明CTS抑制U2OS骨肉瘤细胞的增殖。体外管腔形成试验显示CTS抑制血管生成能力。
CTS可下调U2OS骨肉瘤细胞中VEGF的表达并抑制血管生成。
